Introducing
Your new presentation assistant.
Refine, enhance, and tailor your content, source relevant images, and edit visuals quicker than ever before.
Trending searches
The rate of the reaction will increase as temperature increases. Based on the experimental plan we expect the rate of reaction to increase as temperature increases. We expect our hypothesis to be true and be proven by the experiment. We expect that as the temperature increases the rate of reaction will increase up to a point where the reaction cannot go any faster.Temperature plays a role in why the rate of reaction increases. Molecules are colliding faster so the product is being produced faster.
From this experiment we learned different ways to deal with catechol oxidase. Research helped and it helped us make a decision on which way we wanted to go. We decided to work with temperatures. The 40 degree water bath offered the smallest standard deviation and 20 degree had the highest.
1. Obtain Apple solution by mixing apples, water and the phosphate buffer in a blender
2. Measure out 30g of apples
3. Add phosphate buffer ( 2.5ml per gram of apples)
4. Place in blender, mix well but do not overgrind
5. Pour ending mixture into a funnel with cheesecloth lined around it
6. The liquid obtained will be used as the catechol oxidase
7. Keep extract cold on ice and also covered
8. Heat the solutions in the tube in a waterbath, increase A at 20 degrees, B at 40 degrees, and C at 60 degrees
9. Pipette the extract into cuvettes labeled A B C
Tube A: 2ml phosphate buffer, 0.20ml water, .22ml extract, .28ml 10mM Catechol
Tube B: 2ml phosphate buffer, 0.20ml water, .22ml extract, .28ml 10mM Catechol
Tube C: 2ml phosphate buffer, 0.20ml water, .22ml extract, .28ml 10mM Catechol
10. Shake the cuvettes well to mix
11. Leave the solution out and record observations
12. Use a spectrometer to record the rate of absorption
13. Record rates, mean, and standard deviation
20 degrees water bath
A .591 .700 .755 .804 .837 .883 .916 .963 .972 .1.003
B .330 .331 .338 .355 .350 .351 .357 .372 .377 .383
C .807 .967 1.044 1.089 1.115 1.140 1.150 1.156 1.194 1.206
Mean .576 .666 .706 .743 .767 .791 .823 .830 .847 .864
S.D. .195 .261 .289 .311 .316 .329 .333 .334 .345 .350
40 degrees water bath
A .719 .851 .937 1.030 1.050 1.100 1.137 1.152 1.184 1.189
B .677 .781 .878 .947 .989 1.018 1.052 1.075 1.102 1.136
C .741 .865 .949 1.006 1.044 1.100 1.104 1.140 1.174 1.195
Mean .712 .832 .921 .994 1.03 1.07 1.097 1.12 1.16 1.21
S.D. .027 .037 .031 .035 .027 .039 .035 .034 .037 .032
60 degrees water bath
A .696 .803 .904 .924 .967 .987 1.034 1.072 1.089 1.120
B .730 .847 .934 .973 1.036 1.048 1.081 1.111 1.141 1.161
C .955 1.102 1.174 1.221 1.297 1.304 1.329 1.371 1.391 1.400
Mean .794 .917 1.004 1.039 1.100 1.113 1.148 1.185 1.205 1.227
S.D. .115 .132 .121 .130 .142 .137 .129 .133 .132 .137