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Molecular Fingerprinting
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FREQUENCY
COMB
MOLECULAR FINGERPRINTING
AGENDA
Agenda
- What is molecular fingerprinting?
- Molecular fingerprinting using FTIR
- Molecular fingerprinting using optical spectroscopy
- Set up using spectroscopy
- Applications of molecular fingerprinting.
- Conclusion
INTRODUCTION
INTRODUCTION
Molecular fingerprinting using frequency combs is a technique that uses the unique absorption spectrum of a molecule to identify it. This is done by shining a broadband light source, such as a frequency comb, through the sample and measuring the absorption spectrum. The absorption spectrum is then compared to a database of known spectra to identify the molecule.
ADVANTAGES
ADVANTAGES
One of the main advantages of using frequency combs for molecular fingerprinting is their high spectral resolution and accuracy. Frequency combs are generated by mode-locking a laser, which results in a series of equally spaced, intense spectral lines. The high resolution and accuracy allows for the detection of very small concentrations of a molecule, and also allows for the characterization of complex mixtures of molecules.
FTIR - Fourier transform infrared spectroscopy.
Used incoherent,thermal light and was used to determine molecular structure in spectral region.
TIMELINE
MOVING FROM FTIR TO OPTICAL FREQUENCY COMB SPECTROSCOPY
TIMELINE
1900s
The low brightness retricted visibility, longer propagation and spectroscopic sensitivity.
So optical spectroscopy was introduced as an alternative by providing a unique combination of large spectral bandwidth, high frequency precision and rapid data acquisition
2000s
A coherent, broadband infrared light is produced. This opens up possibilities for infrared scanning probe nanoscopy and potential for achieving broader spectral coverage.
FTIR
SET UP
SET UP
The experimental setup for molecular fingerprinting using frequency combs typically involves a frequency comb laser, a spectrometer, and a sample cell to hold the sample. The frequency comb laser generates the broadband light source, which is shone through the sample cell containing the sample. The spectrometer then measures the absorption spectrum of the light that passed through the sample. This data is then analyzed and compared to a database of known spectra to identify the molecule or molecules present in the sample.
Fig s1
Fig s2
Fig s3
ADVANTAGES
APPLICATIONS AND CONCLUSION
One of the most promising applications of molecular fingerprinting using frequency combs is in medical diagnostics. The high sensitivity and specificity of this technique allows for the detection of very small concentrations of disease markers, such as specific proteins or genetic material, in blood or other bodily fluids. This could enable the early detection of diseases such as cancer, and could also be used to monitor the effectiveness of treatment.
Another important application of molecular fingerprinting using frequency combs is in environmental monitoring. The high sensitivity of this technique allows for the detection of very small concentrations of pollutants or other contaminants in water or air. This could be used to monitor industrial emissions or to detect the presence of harmful chemicals in the environment.
CONCLUSION
CONCLUSION
_______________
In conclusion, molecular fingerprinting using frequency combs is a powerful technique for identifying and characterizing molecules, using their unique absorption spectra. The high spectral resolution and accuracy of frequency combs make them ideal for this purpose. This technique has significant potential for applications in medical diagnostics and environmental monitoring, but also many other fields where identifying specific molecules is important. As technology advances, the accuracy and sensitivity of molecular fingerprinting will continue to improve, making it even more valuable to these and other fields.