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The Processes of Photosynthesis

By: Thomas Wu and Om Bhagia

Photosynthesis

Sunlight is absorbed by the chloroplasts of the cell. The sun is the source of energy in photosynthesis

Photosynthesis

Sunlight

Chloroplast

Light

Reflected Light

Stroma

Chloroplast

Granum

Thylakoid Lumen

Thylakoid Membrane

Lamellae

Thylakoid

Stroma

Sunlight

H+

NADP+ + -> NADPH

H+

ADP -> ATP

Plastocyanin

Cytochrone Complex

ATP Synthase

2 e-

Thylakoid membrane

H+

Re-excited electrons

De-energized electrons

A pair of excited electrons

H+ gradient established

Thylakoid Membrane

Thylakoid Lumen

2 excited electrons for the ETC

Photosystem II absorbs a photon - this causes photoactivation and excites two electrons, propelling them into the ETC. Electrons are replenished through photolysis.

Reaction centre

Photon

Photosystem II

Antenna pigments

Photolysis

+

4e-

O2(g)

Energy

4H+

+

2

Photolysis

Photolysis: energy is needed to break the bonds of the water molecules - this energy comes from the sunlight the chlorophyll has been absorbing. The excess products of the reaction are hydrogen ions (protons) and oxygen gas. Oxygen gas is often excreted as waste, while hydrogen ions will serve as an important part of a later process of photosynthesis.

Stairs analogy: as the two energized electrons travel throughout the electron transport chain, they lose energy - losing energy each step down.

Topic

The released energy from the electrons allow for Hydrogen ions to pass through the thylakoid membrane, establishing the electrochemical gradient.

2 re-excited electrons for the formation of NADPH

Photosystem I takes energy depleted electrons from the ETC and re-energizes them through photoactivation. The electrons are used to reduce NADP+ to NADPH, an important compound for the Calvin cycle later on.

Reaction centre

Photon

Photosystem I

Antenna pigments

Electrons from ETC

ADP -> ATP

ATP Synthase

The electrochemical gradient allows for chemiosmosis across the ATP synthase channel. The high energy protons rushing through the channel provide ample energy for ADP to reduce into ATP

H+

Stroma

carbon dioxide

Stomata

stroma

The Calvin Cycle

2 carbon dioxide

ribulose 1,5-bisphospate

unstable 6C molecule

Carbon Fixing

3-phosphoglycerate

ribulose 5-phosphate

Regeneration of RuBP

Reduction

2 ATP become 2 ADP + phosphate

1,3-bisphosphoglycerate

glyceraldehyde-3-phosphate

ribulose bisphosphate (5 carbon molecule) + carbon dioxide

unstable 6C molecule

(RuBP)

enzyme is ribulose bisphosphate carboxylase

breaks down

2 molecules of 3-phosphoglycerate (each has 3 carbons)

also known as PGA

2 PGA molecules, each phosphorylated into 1,3-bisphosphoglycerate by ATP

thus, 2 ATP used per carbon dioxide

2 NADPH are oxidized in reducing 2 (1,3-bisphosphoglycerate) to 2( glyceraldehyde-3-phosphate)

water also produced

purposes of glyceraldehyde-3-phosphate (G3P)

- provide chemical energy in the cell

- combine to form glucose

- recycled and combined to form RuBP

It takes 5 G3P to make 1 RuBP, but only one carbon can be used at a time to make glucose

takes 6 turns of the Calvin Cycle per glucose molecule

The production of 1 glucose needs

- 12 PGA

- 12 1,3-bisphosphoglycerate

- 18 ATP

- 12 NADPH

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