Cloning Vs. The Biolistic Method

Cloning

Cloning is the process in which one creates an exact genetic copy of an organism, where every bit of DNA is identical in both organisms.

The Biolistic method

The Biolistic Method

The Biolistic method is also known as the gene gun method. This method is used for engineering of the plants. With the use of a gene gun, the gene gun method delivers extra DNA directly into a plant’s nucleus.

Cloning

Cloning

• Petri dish - to put the cells in

• Microscope - to look closer at something

• Sharp pipette - to remove nucleus from egg cell

• Blunt pipette - to hold the cell or somatic cell

• Chemical to stimulate cell division

Biolisitic Method

-Gene Gun

-Petri dish

-Bullet made of gold or Tungsten

-Desired DNA

Cloning

Cloning

1) Isolate a somatic cell from the organism you’re cloning, and an egg cell from egg cell donor.

2) Remove the nucleus from the egg cell.

3) Transfer the somatic cell's nucleus to the egg cell

4)Stimulate cell division until an embryo forms

5)Transfer embryo to the surrogate mother

6)Deliver the clone from the surrogate mother

The Biolistic Method

The Biolistic Method

A Bullet is prepared using metal (preferably gold or tungsten) and is coated in the desired DNA. A pertri dish if also prepared and is filled with the cells being modified, then the bullet is fired using the gene gun into the cell solution, causing the DNA to insert into the cell's nucleus

Cloning

Cloning

Cloning can also be used for medicine like in the stem cell which can make a new heart, bone, reviving endangered or extinct species, cloning livestock.

If we wanted to clone an endangered species we would need

A well-preserved source of DNA from the extinct dinosaur or the extinct species

A closely related species currently living, that could serve as an egg donor and surrogate mother.

In theory we could clone but in reality

Biolistic Method

Biolistic Method

The biolistic method has many uses:

• It is used for pesticide resistance for plants
• It helps research:
• DNA vaccination
• Genetic Immunization
• gene therapy
• tumor or wound healing

Cloning

One big challenge endangered species face is the loss of genetic diversity. When a species has a high genetic diversity there is a better chance that some individuals would have a genetic variation that could help them survive environmental changes. For example diseases.

Cloning

Biolistic Method

• Gene Gun is very expensive (over 20,000 dollars for one process
• It may damage cells
• It may insert undesired DNA into the organisms

Biolistic Method

I think the biolistic method is better because it is easier than cloning. you don't have to reproduce you can just insert DNA into organism

Nooriya

Roy

Roy

I think the Biolistic method is better because, it is able to not only insert DNA directly into the organism without the need of reproduction, but it is also able to mix two organisms DNA, and the resulting organism of this method of genetic engineering is a mix of both organisms unlike cloning, which I see has many more benefits than having an identical organism.