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1.Immobilize a frog by double pithing.
2. Remove skin on one of the frog’s leg.
3. Isolate the gastrocnemius muscle and Achilles tendon.
With a string, tie the Achilles tendon.
4. Cut away the Tibia bone below the knee and cut the femur bone at least 1 cm. above the knee. Clamp the femur using a femur clamp.
5. Tie the string attached to the gastrocnemius muscle to a hook on the force transducer.
6. Adjust the transducer so that the string is taut.
7. Connect copper electrical wires to bipolar electrodes of the electronic stimulator.
8. Wrap the electrical wires at both ends of the gastrocnemius muscle.
9. Keep the muscle wet between the experiments by using Ringer’s solution.
1. SET THE ELECTRONIC STIMULATOR AT 10 V , 1 HZ
2.TURN ON RECORDING CHART AT 5-10 MM/MIN
3.APPLY MULTIPLE STIMULATION TO THE MUSCLE FOR 30-45 S
4.OBSERVE THE RECORDED TRACING
From the muscle stimulus It was observed that, as shown in the graph,
The muscle response increased gradually in the early phases before stabilizing at a later stage.
1.There is increasing availability of Ca2+ in the sarcoplasm
2.Muscle enzyme systems become more efficient because heat is increased as muscle contracts
The concentration of calcium in the sacroplasm increases.
More calcium binds to Troponin C.
Increased strength of muscle contractions
Ready for the next contraction