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Rhodospirillum rubrum
7
Rhodospirillum rubrum
A.K.A Ruby
Introduction
Inroduction
A little information about our bacteria baby
Morphology
Gram-negative
Vibrio to short spirochete
3.0-10 micrometers in length and 0.8-1.0 micrometers in width
Photosynthetic in the light using chlorophyll b using wavelengths 660nm to 680nm
Also uses bacteriochlorophylls (photosynthetic pigments) using wavelengths of 800nm to 925nm
Facultative anaerobe in the dark
Morphology
and
Location
Location and Ecology
Aquatic environments such as lakes, streams, ponds and standing water
Has been found in mud and sewage
Like pH of 6.8-7.2
Temperature range of 22-35ºC
Pathogenesis
Pathogenesis
Rhodospirillum rubrum is not known to be pathogenic to humans or animals
Materials
Agar Cultivation
The bacteria was cultivated using tryptic soy agar (TSA) plates
6.02g of TSA was used for 150ml of water
The media was sterilized in the autoclave on a 15 minute wet cycle
4 plates were prepared
Protocol
Protocol
Using a sterile inoculation loop the bacteria was transferred aseptically from the tube to the plate using the streak plate method
Two plates were streaked and incubated
The plates were incubated at room temperature for 24 hours under the grow lights in the sterile room
Materials
Broth Cultivation
LB broth was used to subculture our bacteria
The broth was pre-prepared and sterilized by Madison and Hillary
Protocol
Using a sterile inoculation loop a non-microsatillite colony was picked up and aseptically transferred to the bottle containing the LB broth
The LB broth was incubated at room temperature on the wrist shaker for 24 hours
Protocol
Materials and Protocol
Freeze Down
In two 1.5ml centrifuge tubes we added glycerol and our bacteria contained in LB broth media in a 1:1 ratio
500µl glycerol
500µl LB broth containing bacteria
The tubes were placed in the freezer at -80ºC
Conclusion
& References
Conclusion
&
References
Ending remarks on our bacteria baby
Conclusion
This bacteria was simple to cultivate and interesting to observe under the microscope
References
https://www.carolina.com/teacher-resources/Interactive/living-organism-care-guide-rhodospirillum-rubrum/tr36405.tr
Munk, C.A., et al. (Jul. 2011). Standards in Genomic Sciences. Complete genome sequence pf Rhodospirillum rubrum type strain (SI2)
https://microbewiki.kenyon.edu/index.php/Rhodospirillum_rubrum