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To determine the concentration of phosphoric acid in a cola of unknown concentration using the application of the Beer-Lambert Law
Introduction
Wavelength - the distance between tops of a wave. Change in wavelength corresponds to change in color and property of light
Absorbancy - the amount of light that is stopped by the solution
Transmittance rate - the amount of light that passes through the solution
Concentration - the amount of substance present in a solution. Expressed as moles of substance/liters of solution.
By creating a phosphoric acid solution with a known concentration and then diluting the solution multiple times we can create a graph of various concentrations and absorbancies at constant wavelength.
Using the graph, we can derive the concentration of the unknown using the equation of the line.
Example Beer's Law Graph
By creating a known solution of phosphoric acid then diluting it, we can determine the concentration of phosphoric acid in a cola of unknown concentration
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Overview
Determine the absorbancies and max wavelength of colored solutions
Determine the absorbancies and transmittance rate of KMnO4 at standard molarity and serial dilutions
Determine the phosphoric acid concentration of Coca-Cola classic using a known phosphoric acid solution of phosphoric acid and AVM.
Create a dilution of coca-cola classic by diluting 4 ml of coca-cola classic in 96 ml of deionized water
Create a blank of coca-cola classic by combining 5 ml AVM and 10 ml of deionized water
Test the diluted coca-cola classic in the Spec-20 for absorbancy at the constant wavelength
Use the equation of the trendline to derive the concentration of phosphoric acid in coca-cola classic
Results
Absorbancies at Different Concentrations of Phosphoric Acid/AVM Solution
Constant Wavelength of 382 nm
Cola Absorbancy: 1.58
Concentration of Cola = (1.58 = 4652.5x + .9548) * 2
Concentration of Cola = .00027 M
Through the application of Beer's Law, we determined the concentration of phosphoric acid in Coca-Cola Classic using a stock solution of phosphoric acid and it's dilution. Using the data collected from the dilutions, we created a scatter plot. Using the equation of the trendline created by the scatter plot, we determined the concentration of phosphoric acid in Coca-Cola Classic
We had a lot of errors in this lab.
Using this research, we can find the concentrations of different substances in unknown concentrations in unknown solutions for forensic science purposes and chemical/industrial purposes
Sample substances found in common solutions: cocaine, citric acid and