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Lab 5. Cellular Respiration

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by

David Ruckle

on 24 October 2016

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Transcript of Lab 5. Cellular Respiration

Citric Acid Cycle (TCA/Kreb's Cycle)
Glycolysis
Start time once you see it "equilibrate". pipet must be submerged between 0.85 and 0.90 mL then push stoppers into test tubes
Lab 5. Cellular Respiration
Exercise I. Effects of Various Compounds on CO2 Production during Ethanol Fermentation (YEAST)
Each compound has an effect on anearobic respiration.
Take care in adding the various compounds.
Poke hole in parafilm, heat the test tubes in the water bath for 40 min.
Move onto Exercise 2 while you wait.
Question 5 (pg. 79): "What does it smell like?"
Exercise III. Production of CO2 in Aerobic Respiration (FISH)
CO2 combined with water makes carbonic acid (H2CO3)
Aerobic and Anaerobic Oxidation
Cellular Respiration:
Energy stored in chemical bonds of ATP (adenosine triphosphate)
Acetyl CoA enters mitochondrial matrix
Oxidized to produce some ATP
Reduces NAD+ to NADH and FAD+ to FADH2
electron carriers and donate electrons to ETC (electron transport chain)
Anaerobic Respiration: without oxygen
Set Up
More respiration--> More CO2--> More H2CO3--> More acidic--> Lower pH
Measure respiration indirectly via titrations
add NaOH until SAME SHADE OF PINK!
DO NOT ADD ANYTHING TO BEAKERS WITH THE FISH OR ELODEA IN IT. THEY WILL DIE!
Final Remarks

Exercise II (Peas)
Groups of 4
Cap KOH pellets tightly or else they will lose potency and you will lose points!
Return all materials to the table

Exercise III (Fish and Elodea)
Groups of 2
ask TA to obtain fish and Elodea
Ask TA to return fish after allowing 15 minutes of respiration (limited fish. they keep dying)
Keep count of drops of NaOH during titration exercise
Exercise II. Oxygen Consumption in Aerobic Respiration (PEAS)
When it says "equilibrate", it means water in pipet is level with water level in beaker
Overview
Breaking apart chemical bonds releases energy for cellular use
using up energy stored in cells
Oxidative decarboxylation
pyruvate (3-carbon) -----> Acetyl CoA (2-carbon)
via oxidation
"de-carboxylation" (carboxyl group comes off, 3-carbon molecule becomes 2-carbon )
occurs in mitochondria
First part of cellular respiration
Occurs in cytosol
Glucose (6 carbon) --> pyruvate (3-carbon)
Presence of oxygen (aerobic)
or
Absence of oxygen (anaerobic)
Electron Transport Chain (ETC)
Occurs in inner mitochondrial membrane
Energy from electrons (NADH and FADH2) creates proton gradient via coupling a proton pump
Gradient powers ATP production by ATP synthase complex
OXYGEN is the final electron acceptor
Oxygen is reduced to water
Aerobic Respiration: with oxygen
cap KOH pellets or whole lab will lose points!
Carefully follow setup instructions!!
shake out water out of pipets before using
Discussion Section
Written on lab #5 (Respiration)
Check and follow rubric- At the end of the manual under Files section on BIOL 111 on Canvas!
Next week we will talk about the full lab report to come!
Full transcript