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Journal Club 1: How to Read a Journal Article
Transcript of Journal Club 1: How to Read a Journal Article
Should give an idea of what was done and results
Authors and Affiliations
First author: did most of the work
Last author: head of the lab (PI)
Overview of the entire article. Some background, methods, results and analysis.
This can be the toughest part of the paper to get through.
Section 1: Introduction/Background
Information on what the paper is about
What is already known about the topic?
What are the researchers trying to find out?
Results may be briefly mentioned.
Section 2: Materials and Methods
How did the researchers perform their experiments?
EVERY experimental detail is in here.
MUST have enough detail for the exact studies to be replicated by others.
This section is not overly important for our purposes, but you may want to look it over when you are picking a technique to review.
Section 3: Results
Results of the experiments.
All the data are included here.
Some data may be in supplemental information (online)
Even More Results
Data, Data, Data
Section 4: Discussion/Analysis
What do all the data mean?
How do they fit into the larger body of data?
What avenues of further study does this research open?
This section of the paper is open to debate.
Do the researchers misinterpret their findings?
Are their conclusions fully supported by the data?
Section 5: References/Citations
Citations of previous research discussed in the paper.
Evidence that science does not occur in a vacuum, but always builds on what was done before.
This section is not overly important for our purposes.
HCMV Major Immediate Early Region
IE2 Poly A
IE1 Poly A
(MOI = Multiplicity of Infection)
The acidic domain of IE1 plays a role in viral replication
Chromatin tethering of IE1 is dispensable for viral replication
C-Terminal phosphorylation is dispensable for viral replication
How to Read a Journal Article
Areas for Further Study
IE1 role in latency
Role of IE1 phosphorylation
Glutamic Acid (E)