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Determine the growth rate constant and generation time
Where the slope is equal to the growth rate constant
=(〖〗_ )/
write the equation
Calculate cell Growth
Cell division occurs exponentially
Equation that describes cell growth during the exponential phase assuming a constant rate of cell division
=_ 2^
Where n = the current or final number of cells
n_o = the initial number of cells
d = the number of cell divisions
Phases of cell growth
Lag phase - adjusting to new environment
Exponential growth - population doubles each division
Stationary growth - growth slows, population limit, exhausted food
Death phase - toxins and waste in excess
1. Macroscopically
2. Microscopically
3. Impedence
4. Spectrophotometrically
Spectrophotometrically
Macroscopically
optical density
colony counting
Impedence
Microscopically
1. Examine growth kinetics of E. coli
2. Track growth of culture at 3 different time points using macroscopic and spectrophotometric methods
3. Create a growth curve with the data collected
- Find the growth rate constant and generation time
4. Graph both methods of tracking growth for correlation