Introducing 

Prezi AI.

Your new presentation assistant.

Refine, enhance, and tailor your content, source relevant images, and edit visuals quicker than ever before.

Loading…
Transcript

Kinetics of Cell Growth

Determine the growth rate constant and generation time

Where the slope is equal to the growth rate constant

​​=(〖​​​​​​〗_​​​​ ​​)/​​

write the equation

Calculate cell Growth

Cell division occurs exponentially

Equation that describes cell growth during the exponential phase assuming a constant rate of cell division

​​=​​_​​ 2^​​

Where n = the current or final number of cells

n_o = the initial number of cells

d = the number of cell divisions

Growth Kinetics

Phases of cell growth

Bacterial division

Lag phase - adjusting to new environment

Exponential growth - population doubles each division

Stationary growth - growth slows, population limit, exhausted food

Death phase - toxins and waste in excess

Methods commonly used to Monitor Cell Growth

1. Macroscopically

2. Microscopically

3. Impedence

4. Spectrophotometrically

Spectrophotometrically

Macroscopically

optical density

colony counting

Impedence

Microscopically

Today's experiment

1. Examine growth kinetics of E. coli

2. Track growth of culture at 3 different time points using macroscopic and spectrophotometric methods

3. Create a growth curve with the data collected

- Find the growth rate constant and generation time

4. Graph both methods of tracking growth for correlation

Learn more about creating dynamic, engaging presentations with Prezi