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What is GFP?
How can we use it?
How does GFP help us?
T.Hanks for your attention!
Luciferase
488nm
507nm
target-gene
promoter
ATG
TAA
TGA
TAG
promoter
GFP-gene
target-gene
TAA
TGA
TAG
Step 1: sequence target protein
Questions:
Step 2: introduce GFP
Which advantages does fluorescence bring jellyfish?
Step 3: gene expression
Step 4: Translation
How would you research two different proteins at the same time ?
Step 5: inducing fluorescence
by Moritz Klein & Fritz Herre