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How to mark proteins using GFP

Sources:

  • Iruntheinternet.com: http://iruntheinternet.com/lulzdump/images/Tom-Hanks-thanks-t.hanks-boat-12711996690.jpg?id= [Last access:13.12.2018]
  • Wikipedia.org: https://en.wikipedia.org/wiki/Green_fluorescent_protein ; https://en.wikipedia.org/wiki/Aequorea_victoria [Last access:13.12.2018];https://ka-perseus-images.s3.amazonaws.com/d3027730681b4cda353ea0e3895b6670ce9024ea.png [Last access:13.12.2018];
  • Docplayer.com:https://docplayer.org/docs-images/63/49291667/images/15-1.jpg [Last access:13.12.2018]
  • mirusbio.com: https://duckduckgo.com/?q=Crispr+cas9+simple&atb=v144-1a_&iar=images&iax=images&ia=images&iai=https%3A%2F%2Fwww.mirusbio.com%2Fassets%2Ffigures%2Fcrispr-cas9-genome-editing.png [Last access:13.12.2018]

What is GFP?

How can we use it?

How does GFP help us?

What is GFP?

T.Hanks for your attention!

Aequorea victoria

Green Fluorescent Protein

Luciferase

488nm

507nm

How can we use it?

Possibilities of GFP

Procedure

  • reporter for protein localisation
  • gene activity

target-gene

promoter

ATG

TAA

TGA

TAG

promoter

GFP-gene

target-gene

TAA

TGA

TAG

Step 1: sequence target protein

Questions:

Step 2: introduce GFP

Which advantages does fluorescence bring jellyfish?

Step 3: gene expression

Step 4: Translation

How would you research two different proteins at the same time ?

Step 5: inducing fluorescence

by Moritz Klein & Fritz Herre

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