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HPLC FLOWCHART
2
Transcript
HPLC FLOWCHART
Start
By Chris Schumacher
Research The Compounds
Find the following information about the analytes:
- pKa
- Solubility
- Polarity
- Structure
Set initial parameters
Set the following initial parameters:
- 100%B
- Column with suitable ligands - if known
- Choose suitable solvent - if known. if not known choose cheapest solvent
- pH = 3 -
- Isocratic
Run HPLC and record data
Use Normal Phase Chromatography
If Tr < 4 Mins
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