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The occurrence of reverse mutations helps distinguish point mutation from large mutation from large mutational events like deletions.
Ionizing Radiation
High-energy radiation capable of producing ionization in substances through which it passes. It includes nonparticulate radiation, such as x-rays, and radiation produced by energetic charged particles, such as alpha and beta rays, and by neutrons, as from a nuclear reaction.
These are mutagenic agents that are caused by chemicals such as chloral hydrate, acenaphthene, nitrous acid
A mutation of an existing mutant gene that restores it to its previous form or the reversion of a mutant to the original phenotype.
Mutation may occur spontaneously or may be induced. The mutagenic agents are varied and their effectivity may be specific in certain cases or general in other cases.
Removal of young shoots or axillary buds will induce callus formation.
http://dictionary.reference.com/browse/reverse+mutation
http://www.apsnet.org/edcenter/advanced/topics/Pages/ReverseGeneticTools.aspx
http://www.genpharmtox.de/downloads/AssaySheetAMESTEST.pdf
http://www.slideshare.net/DrRiteshshiwakoti/mutagens
The naturally occuring and the experimental allopolyploids are protects of interspecific or intergeneric hybridiztion.