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Cane toads will be the main source of the subject of this study. The paratoid glands of these animals produce a toxic white fluid called Bufotoxin. Bufotoxin acts as a defense mechanism of these toads to protect themselves from predators. It will be used as a source of Epinephrine or Adrenaline.
1.Was the epinephrine and bufotoxin separate substances in the toad’s body?
2.Was the effect of bufotoxin and epinephrine the same?
3.Was the extraction of epinephrine in bufotoxin possible?
1.The two substances (Bufotoxin and Epinephrine) are not separate substances.
2.The effects of the substances are the same.
3.The extraction of epinephrine from bufotoxin is possible.
Verification if Bufotoxin contains Epinephrine thru Laboratory Testings.
Experiment 2
Experiment 1
Bufotoxin
Controlled
Bufotoxin
Epinephrine
Three white mice with 0.3 mL of Epinephrine
Three white mice with 0.3 mL of Bufotoxin
Two white mice with no dose of anything
Three white mice without any dosage of Bufotoxin
Three white mice with 0.3 mL dosage of Bufotoxin
Three white mice with 0.2 mL dosage of Bufotoxin
Three white mice with 0.1 mL dosage of Bufotoxin
Observation Time
Proper Treatment
Final Analysis
INPUT
Bufotoxin
PROCESS
Two different experiments will be conducted in this study. For the first experiment, three treatments will be assigned to eight samples of white mice. For the second experiment, four treatments will be assigned to eleven samples of white mice.
a.Recording of initial behaviours, body condition and physical appearance.
b.Preparation of all needed materials.
c.Testing
d.Recording of observations and data.
e.Checking the effectively of product.
f.Final Analysis
OUTPUT
Bufotoxin as source of Epinephrine