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Pax2a Expression During Zebrafish Development
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Transcript
Question
How is pax2a expressed in zebrafish embryos at 24 and 48 hours post fertlization?
Results: 24 hpf embryos
Embryos treated with antisense probe show staining in:
- Yolk Sac
- Midbrain-Hindbrain Boundary
- Spinal Cord Neurons
- Otic Vesicle
Antisense
Sense
Dorsal
Lateral
Pax2a Expression During Zebrafish Development
Background
- Transcriptional regulation
- Function of gene
- Where is it expressed?
- In Situ Hybridization
In Situ Hybridization
24 vs. 48
Results: 48 hpf embryos
Embryos treated with antisense probe showed staining in:
- Midbrain-Hindbrain Boundary
- Hindbrain
- Optic Nerve
- Spinal Cord Interneurons
- Otic Vesicle
Discussion/Conclusion
- Pax2a expression is localized in nervous and sensory tissue.
- Consistent with other results (Thisse et al., 2001).
48 hpf
Long-pec (48hpf)
24 hpf
Pharyngula: Prim-5 (24hpf)
Antisense
Future Directions
- Compare pax2a expression at earlier and later stages in development.
- Evaluate pax2a expression in mutants with nervous system defects.
- Implement ISH in future projects.
Lateral
Dorsal
Sense
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