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Presentation - Analytische Messtechnik

„CULTIVATION OF ESCHERICHIA COLI BAKTERIA

AND EVALUATION OF SELECTED SENSORTECHNICAL

METHODS FOR DETECTION OF ESCHERICHIA COLI

BAKTERIA IN SIMPLE MATRICES"

Tom PRSTEK

01405108

Supervisors: Christine UNGER

Mathias PETSOVITS

Martin WERNER

Birgit BRAUER

Atomic Force Microscopy

Cantilever with tip

scaning surface

- taping mode

- contact mode

QCM & Electropolymerisation

Quartzcristallmicrobalance Preparation

Gold-oil suspension

Spraygold

Bacteria application

Electropolymerisation

scaning a target

using an electron

beam

Detection of

scattered electrons/

secondary electrons/

auger electrons/

X-rays

Scaning electron microscopy

Stamp-

technique

Polymerisation

Polymerisation and Stamping with bacteria

Microscopic image

before

SEM image

after

Polymer-Beads with embedded Bacteria

QCM

&

Electropolymerisation

Stamp-technique Polymerisation

Methods

Washing incomplete

QCM & Electropolymerisation

resulting in formation of beads with embedded Bacteria

Problematic washing

QCM

- Electropolymerisation

Stamp-technique Polymerisation

Polymer-Beads with embedded Bacteria

Atomic Force Microscopy

Scaning Electron Microscopy

Styrene-Divinylbenzene- polymerisation mixture

together with aquaeus

Bacteria forming emulsion

Polymer covalently bonded to the Glycocalix of Bacteria

Polymer-Beads

with embedded

Bacteria

Tom PRSTEK

Derivatisation of glas - surface

Immobilising of Bacteria

Vinyl endgroups on glas to bind

the polymer covalently

Derivatisation of glas - surface

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