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ICGEB Course on Bacillus thuringiensis 2018
1
ICGEB Course on Bacillus thuringiensis 2018
CBS, Sfax, Tunisia
Conclusion
- We report the biological and genome properties of virulent and temperate B. megaterium phages.
- The first comparative whole genome nucleotide sequences analysis, which reveal the clustering and sub-clustering of B. megaterium phages.
- The study attempts to present the most comprehensive phylogenetic analysis of the Bacillus phages, revealing a robust relationship between the phage families and packing strategies and supporting the fact that the distribution of tRNA genes in the Bacillus phage is specific to sub-clusters.
- Our screening of the 87 Bacillus phages genomes for integrases and tRNA genes revealed that 66.7% of the 66 lytic Bacillus phages lacked tRNA genes while 25% of the 20 temperate Bacillus phages contained tRNA genes.
- We do not agree with the assumption that lytic bacteriophages are more likely to contain tRNA genes than temperate bacteriophages.
the magical part
Dry lab
Wet lab
BM5 phage (267.273 reads) = 165 kbp
BM10 phage (288.254 reads) = 165.2 kbp
Raw NGS Reads
FastQ
Adapters and Quality Trimming
@HWUSI-EAS582_157:6:1:1:1501/1
NCACAGACACACACGAACACACAAAGACATGCCCATATGAAGAT
+
%.7786867:778556858746575058873/347777476035
@HWUSI-EAS582_157:6:1:1:1606/1
NCTGGCACCTTGATTTTGGACTTCCCAGCCTCCAGAACTGTGAG
+
%1948988888798988366898888648998788898888588
Annotation
Quality Control
Assembly
Visualization
BM5 = 225 ORFs
BM10 = 283 ORFs + 17 tRNAs
Our Story
Characterization and Comparative Genomics Analysis of Virulent and
Temperate Bacillus megaterium Bacteriophages
Stain: gram (+).
Shape: rod.
Special: can live in extreme environments.
Habitat: very common in soil and vegetation.
pathogenicity: nonpathogenic.
Use: a soil inoculant for agriculture.
Bacillus megaterium
Industrial
Biodetection
Therapeutic
Biocontrol
Thanks for your attention
This presentation is funded by project Centre for research of pathogenicity and virulence of parasites
r.n° CZ.02.1.01/0.0/0.0/16_019/0000759
Sarcinochrysis sp.
Cafileria
Classic Assembly
De novo Assembly