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CIMac pDNA column

MJ

Marko Jarc

Transcript

Thank you for your attention

BIA Separations

pDNA purification process

CIMac pDNA analytical column

...specifically developed in order to monitor a plasmid DNA quantity and purity throughout the downstream processing of plasmid DNA

C4 ELUTION

BACTERIAL LYSATE

Analytical chromatography

  • Column: CIMac pDNA Analytical Column
  • Equilibration buffer: 200 mM Tris, pH 8.0
  • Elution buffer: 200 mM Tris, 1M NaCl, pH 8.0
  • Method: 60 to 75 % buffer B within 100 CV
  • Flow rate: 1 ml/min
  • UV detection at 260 nm

DEAE ELUTION

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