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Transcript

Things That Could Go Wrong

With our experiment we saw that grapes have catechol oxidase like potatoes do. We figured that it would go on the same path as it did with the potato extract. We felt that the unknown b would speed up the reaction, whereas the unknown c would slow down the reaction.

What happened

Our experiment did what we expected it to do. With the grape extract, our absorbency started really low compared to the potato. But the unknowns did what they were supposed to do, The extra phosphate buffer sped up the reaction where as the thiourea slowed down the reaction.

Results & Data

Experimental Design

What Would we Want to do Differently!

2

If we were to do this experiment again, we would want to use Victoria grapes. These grapes are grown in South Africa and contains Polyphenoloxidase (PPO), which is a copper- containing oxidoreductase that reacts with oxygen. These grapes also have more catechol than the grapes that we used. So we would like to use these grapes to see if the absorbency would increase compared to the grapes we used.

In our experiment, we used grapes as our catechol extract. With this we used a phosphate buffer, and a crude extract as our control group. With our experimental groups we used unknown B, which turned out to be a different phosphate buffer, and unknown C, which was thiourea to determine what would happen. We had three different groups containing three trials in each group. Group a was our control group. Group b had the second phosphate buffer in it. and Group c had the thriourea.

Introduction

1

Catechol oxidase is an enzyme that catalyzes the oxidation of catechol. Catechol oxidase contains copper and in a reaction with oxygen and catechol, produces a benzoquinone substance. This substance is the cause of the coloration in fruits and vegetables that have been sitting out for a while.

Hypothesis

Sources

Eicken, Krebs, Sacchettini. (1999). Catechol Oxidase structure and activity. Current Opinion in Structural Biology, 9(6), 677-683.

If we use grape extract with the unknown B & C, it will have the same effect as it did with the potato extract. Meaning,the unknown B will speed up the reaction whereas the unknown C

will slow down the reaction.

Rapeanu, Loey, Smout, Hendrickx.(2004).Biochemical Characterization and Process Stability of Polyohenoloxidase extracted from Victoria grape. Food Chemisty, 94, 253-261.

The oxidation of Catechol to Benzoquinone in Grape Extract.

By Veronica Szweda & Laura Kraus

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