Introducing 

Prezi AI.

Your new presentation assistant.

Refine, enhance, and tailor your content, source relevant images, and edit visuals quicker than ever before.

Loading…
Transcript

Factors Affecting Enzyme Activity

Investigation of urease enzyme and indicator

Question 2.

Can you predict what color of the solution will be if the urease enzyme convert urea to ammonia?

Put 3ml of red cabbage and 2 drops of critic acid

Question 1.

Why is it important to have three reference tubes?

It changed from purple to pink

Put 3ml of red cabbage and 2 drops of baking soda

Put 3ml of red cabbage and 2 drops of distilled water

It changed from purple to blue

Color get lighter

Investigating the effect of urease from soya beans on urease

Add 2ml of red cabbage indicator, Add 2ml of distilled water, and Add 2ml of soya suspension

Question 2.

Question 1.

Why do we need to add an enzyme into a test tube without urea even though we can predict that no enzyme activity will occur?

How does temperature affect the urease activity? How do you know?

The color changed to light purple.

Add 2ml of red cabbage indicator, Add 2ml of urea solution

Question 1.

What are the purposes of procedure 2?

The color not changed

Add 2ml of red cabbage indicator, Add 2ml of urea solution, and Add 2ml of soya suspension

Question 3.

Describe the appearance of the denatured urease enzyme in the test tube.

Question 2.

The color changed to green.

From your results, what temperature would you use and avoid if you have to conduct an experiment using urease enzyme and why?

enzyme and why?

Conclusion

Effect of temperature on urea

If a temperature is too low, there can be no noticeable reaction rate since the enzyme is operating at a temperature far below its optimum. If the temperature at which the enzyme is operating at is well above 100oC, then thermal deactivation can occur. Therefore, it is important in many process design and manufacturing levels to have the correct temperature of reaction. If the reaction temperature is too high, the enzymes will eventually deactivate in an irreversible way and thus halting the reaction from taking place.

Boil water

In ice bath

Beaker on hot plate 97°C

At room temperature

Beaker on hot plate 80°C

Learn more about creating dynamic, engaging presentations with Prezi