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Can you predict what color of the solution will be if the urease enzyme convert urea to ammonia?
Why is it important to have three reference tubes?
Why do we need to add an enzyme into a test tube without urea even though we can predict that no enzyme activity will occur?
How does temperature affect the urease activity? How do you know?
What are the purposes of procedure 2?
Describe the appearance of the denatured urease enzyme in the test tube.
From your results, what temperature would you use and avoid if you have to conduct an experiment using urease enzyme and why?
enzyme and why?
If a temperature is too low, there can be no noticeable reaction rate since the enzyme is operating at a temperature far below its optimum. If the temperature at which the enzyme is operating at is well above 100oC, then thermal deactivation can occur. Therefore, it is important in many process design and manufacturing levels to have the correct temperature of reaction. If the reaction temperature is too high, the enzymes will eventually deactivate in an irreversible way and thus halting the reaction from taking place.