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Application of monoclonal antibodies in pregnancy tests
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Monoclonal antibodies
Monoclonal antibodies?
An antibody produced by a single clone of cells or cell line and consisting of identical antibody molecules.
How they're made
Application of monoclonal antibodies in pregnancy tests
How it's done
By: Krushang
-Monoclonal antibodies specific to hCG are conjugated to an enzyme that changes the colour of a dye.
-A second set of monoclonal antibodies specific to hCG are immobilized to the dye substrate.
-If hCG is present in urine, it will interact with bot sets of monoclonal antibodies.
-When both sets of antibody are bound to hCG, the enzyme is brought into physical proximity with the dye, changing its colour.
-A third set of monoclonal antibodies will bind any unattached enzyme-linked antibodies, functioning as a control.
Pregnancy testing
The process behind it
Why it's possible
Pregnancy tests use a process called ELISA (enzyme-linked immunosorbent assay) to identify a substance through a colour change.
Monoclonal antibodies can be used to test for pregnancy through the presence of human chorionic gonadotrophin (hCG) in the urine. The hormone hCG is produced by women during foetal development