Introducing
Your new presentation assistant.
Refine, enhance, and tailor your content, source relevant images, and edit visuals quicker than ever before.
Trending searches
NATURAL
+ cell adhesion sites
+ cell-mediated degradation
- batch-to-batch variation
- limited range of properties
- sterility / endotoxins
SYNTHETIC
+ tailorable
+ reproducible
+ GMP
- lack of biological
functionality
- expensive
Klotz, ..., Melchels 2016 Trends in Biotechnology
polyacrylamide
PEG-DMA
PEG-PLGA
pHPMAm-
lactate-PEG
DNA-polypeptide
poloxamer-DA
proteins
collagen
gelatin
fibrin
elastin
silk
polysaccharides
alginate
aggarose
methylcellulose
carrageenan
chitosan
hyaluronic acid
HYBRID
gelMA/gellan
HA-SH + PEG-A
HA + dex-HEMA
PEG-DMA + gelMA
PEG-DA + alginate
Benya PD, Shaffer JD. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels.
Cell 1982;30:215–24
Dunn JCY, Yarmush ML, Koebe HG, Tompkins RG. Hepatocyte function and extracellular matrix geometry long-term culture in a sandwich configuration.
FASEB J 1989;3:174–7.
dECM
fat
cartilage
heart
Schuurman & Levett, ..., Melchels et al 2013 Macromolecular Bioscience
cancer
OV-MZ-6 ovary cancer cell line
Crosslinks can be chemical or physical
cartilage
Applications
Levett, Melchels et al 2013 J Biomed Mater Res A
Lim, ..., Melchels et al 2018 Biofabrication
Hochleitner et al 2015 Biofabrication
Melchels & Blokzijl et al. 2016 Biofabrication
PCL, PCL and ..... PCL
1) yielding
2) shear-thinning
gelatine-methacryloyl (gelMA)
MACRO
MICRO + MACRO
Hinton et al. 2015 Science Advances
Bhattacharjee et al. 2015 Science Advances
Keriquel et al 2010 Biofabrication
Mouser, Melchels et al 2016 Biofabrication
Gladman et al. 2016 Nature Materials
Chang et al. 2011 J Biomed Mater Res B Appl Biomater.
metals
Ti
CoCrMo
Ti6Al4V
316SS
Xu et al 2009 Biofabrication
Visser, Melchels et al. 2015 Nature Communications
Kämmerer, Melchels et al 2014 Acta Biomaterialia
hydrogels
PEG-DA + alginate + nanoclay
Gellan gum:
Melchels et al. 2014 Journal of Materials Chemistry part B
Dietmar Hutmacher
QUT
Dirk Grijpma
UTwente
Jos Malda
UMC Utrecht
Mannoor et al. 2013 Nano Letters
PETA.org
blue = 20M cells/mL
red = 60M cells/mL
Luo et al, 2013 J Mater Chem B
Kämmerer, Melchels et al 2014 Acta Biomaterialia
bioactive glass
Melchels & Blokzijl et al. 2016 Biofabrication
Paxton, ..., Melchels et al. 2017 Biofabrication
* denatured extracellular matrix
* cell adhesion sites
* enzymatically degradable
* non-immunogenic
* cheap and widely available
* thermosensitive
thermosets
PCL
PDLLA
PTMC
PPF
Schuurman et al. 2011 Biofabrication
Caliari et al 2016 Nature Methods
Pati et al. 2014 Nature Communications
ceramics
TCP
HAP
CaSiO3
Al2O3
SiO2
Mg3(PO4)2
Kesti & Müller et al. 2015 Acta Biomaterialia
MICRO
endothelial cells in Matrigel
making a capillary network
Kubota et al 1988 Journal of Cell Biology
Klotz, ..., Melchels et al European Cells and Materials (submitted)
graphene
Fluorescence Recovery After Photo-bleaching (FRAP)
Loessner, ..., Melchels et al 2016 Nature Protocols
collagen II aggrecan collagen I
explainingthefuture.com
Spiller et al 2011 Tissue Eng Part B Rev
Melchels et al. 2014 J Mater Chem B
Kolesky et al. 2014 Advanced Materials + 2016 PNAS
thermoplastics
PL(G)A
PCL
PTMC
PolyActive
PHB(V)
chitosan
gelMA PEG-MA
HA-MA AL-MA
54x
Malda et al. 2013 Advanced Materials
3) photo-curing
Levett, Melchels et al 2014 Acta Biomaterialia
Visser, Melchels et al. 2015 Nature Communications