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Chromatography

Chromatography: A set of laboratory techniques for the separation of mixtures, or colors

Used in the real world to isolate new compounds and sequence DNA

What we found

  • The more rings there are on the pieces of chalk during our chromatography experiment, the farther the alcohol the sharpie pigments will travel up the chalk
  • The more ink we have on the chalk, the farther the pigment will rise

Procedure

1. We filled a aluminum dish with rubbing alcohol

2. We created a ring on three pieces of chalk and created two rings on three other pieces with a sharpie

3. We placed the pieces of chalk overnight into the dish and let the chalk absorb the alcohol

Observations

  • Alcohol is absorbed by chalk and moves up through it
  • A chemical reaction occurs with the colors and causes the pigments to rise showing the colors within the original color
  • When we picked the chalk up, the chalk felt like a wet sponge
  • After we left the chalk overnight, the colors became more subdued because of evaporated pigments

Picture

Below is a picture of our experiment after a night in Mr. Kennedy's room:

Our Results

Chalk w/ one ring:

1. 9 mm

2. 17 mm

3. 10 mm

Average distance Sharpie pigments traveled:

12 mm

Chalk w/ two rings:

1. 12 mm

2. 25 mm

3. 15 mm

Average distance Sharpie pigments traveled:

~17 mm

Chalk Chromatography

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