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reagent

  • acetic anhydrite
  • conc. sulfuric acid

purpose:

  • to react with the C3 Hydroxyl group of the cholesterol
  • used as a colorimetric test to detect cholesterol

Liebermann-Burchard Test

principle:

OXIDATION!

Objectives: determine the prescence of unsaturated sterols, in this test cholesterol, in the given sample

by: Von Parreñas

compund responsible for green color: hydroxyl group of cholesterol reacting w/ reagent

group of lipids identified: steroids/cholesterol

importance of dry equipments: To avoid dilution of solution or if the material used in the

experiment is conc., it may react with water.

equation:

results:

residue C ---------- blue-green solution

vegetable oil------ emerald green solution

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