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Group 1: Anessa Yuriar, Jeffrey Walker, Samantha Reynolds, Ethan McDaniel
3 long phenotype: body length is longer and thinner (Lon)
2 blister phenotype: fluid filled blisters appear on cuticle (Bli)
3 dumpy phenotype: worms are shorter and stouter (Dpy)
2 uncoordinated: deviations in normal movement (Unc)
Blister
Dumpy
Long
Uncoordinated
8. After lifecycle is complete, the worms have become adult worms after about 4-5 days.
9. As adults, transfer 5-7 worms from each 1-5 plates of P0-A and each 1-5 plates of P0-B to corresponding labeled plates for F1-A (1-5) and F1-B (1-5).
10. 2 days later, the adult worms will have laid eggs in both groups of plates. Remove adults and flame.
11. Allow a day for F2 to hatch then screen for mutagens.
12. Transfer mutagens to their own plate.
4. With eggs laid, transfer the 5 adults worms to new NGM plates like in step 2, but labeled P0-B1,2,3,4,5 all containing E.coli
5. Allow 12-24 hours to pass for new eggs on new plates
6. With eggs on B plates, remove adult worm from each of the plates and flame
7. Allow F1 generation to hatch on A & B plates
**NOTE: Since plates A are ahead of B, make sure enough E.coli is constantly present for the worms to eat or they will starve.
1. Decide which type of phenotype the group wants to base the forward screen on
2. Provided: P0 mutagenized worms from EMS, transfer 5 adult C. elegans and placed each on fresh, new nematode growth medium (NGM) plates labeled P0-A1,2,3,4,5 all containing E. coli (worm's source of food)
3. Allow 12-24 hours to pass for the worms to reproduce and lay eggs
If we use mutated Caenorhabditis elegans from stock and breed them, can we find roller phenotype in the F2 offspring? Or any other mutations?
Basically... how does the introduction of a genetic mutation lead to an understanding of a biological process?
No rollers :( BUT.....
we found some other awesome mutagens! We've learned that through this screen a phenotype can explain a lot about the organism's biological process such as movement, shape, action, etc. This screening process is useful in understanding how genetics influence biological processes and further studying may be used to better understand the human body and beyond.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1214229/
http://wormclassroom.org/c-elegans-phenotypes
http://jcs.biologists.org/content/117/22/5209/F7.expansion.html
Rollers video and Uncoordinated video: link attached to videos
Genetics Laboratory Manual Fall 2013
Thank you for your time.