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Background

  • Mobile elements-segments of DNA that are able to "jump" from one adult organism to another.
  • 1st discovered by Barbara McClintock and found in ALL organisms
  • ~50% of the Human genome is composed of transposable elements
  • 3 types of transposons
  • Today's focus: DNA-only cut and paste elements

TcBuster and SPINon are highly active in mammalian cells

Mobile DNA Elements

"A resurrected mammalian hAT transposable element and a closely related insect element are highly active in human cell culture"

Purpose: To characterize two hAT elements, Space Invaders (SPIN) and TcBuster.

  • hAT is a superfamily of eukaryotic transposable elements
  • SPIN and TcBuster are part of a subfamily known as Buster elements
  • SPIN- currently inactive, but were once highly active in several vertebrate genomes. A "resurrected" SPIN element (highly active in human cells) was produced and used for this study.
  • TcBuster- Currently active . From Tribolium castaneum, a red flour beetle. Also happens to be highly active in human cells.

Presented by: Taylor Neff

Integration preferences

Inducible Transposition

Questions

and

Comments

Transposases and their products

Work Cited

Li, X., H. Ewis, R. H. Hice, N. Malani, N. Parker, L. Zhou,

C. Feschotte, F. D. Bushman, P. W. Atkinson, and N. L. Craig. "A Resurrected Mammalian HAT Transposable Element and a Closely Related Insect Element Are Highly Active in Human Cell Culture." Proceedings of the National Academy of Sciences 110.6 (2013): E478-487. Fusion. Web. 17 Feb. 2015.

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