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Processes of phagocytosis and exocytosis in Tetrahymena when 4μL latrunculin A is added

Introduction

Latrunculin A

Protein: Actin

Future Experiments

Hypothesis

4 μL Lat A was used for the new experiment

A large amount of Lat A will inhibit the cytoskeleton of the Tetrahymena by blocking the active sites of the protein actin. If enough Lat A is added, function of the cytoskeleton will ceases entirely.

  • Find estimate amount of Lat A needed to completely inhibit phagocytosis
  • See if colchicine will inhibit exocytosis

Discussion

Revisiting Hypothesis

Controls Explanation

Exocytosis

Goals Accomplished?

Our hypothesis predicted one of the outcomes, but not in the way we expected

Sort Of...

Everything Else

(units, xy axis, etc.)

Phagocytosis

  • Actin microfiliments were inhibited in the cytoskeleton by Lat A
  • Exocytosis decreased
  • Phagocytosis remained the same

Units: average number of vesicles/minute

X-axis: minutes

Y-axis: average number of vesicles

Standard Error Bars

Results

Red Vesicles

Black Vesicles

Methods

What We Did The Same

In summary... we did everything the same from the week before, except for adding water during step 5

What We Did Differently

Lat A

4 μL of Latrunculin A was added to Tetrahymena cells before they were "fixed"

Other Controls

When compared to the experiment last week...

  • 0.5 mL of Tetrahymena
  • 0.5 ml of 1% india ink
  • incubation time
  • 500 μL of 2% proteose peptone
  • 0.2 carmine
  • 25 μL of 3% gluteraldehyde

Lab Members: Florentyna Kwasniak, Grace Dostie, Jessica Duncan, Jazmin Wright-Zornes

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