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Hyperthyroidism and Cation Pumps in Human Skeletal Muscle

Results

Hyperthyroid: 6,555 +/- 604 pmol/g

Euthyroid: 5,212 +/- 1,580 pmol/g

Results

Healthy Control:

4,523 +/-1,311 pmol/g

P=0.04

vs.

Euthyroid

Hyperthyroid

Paired t-test:

P=0.0005

Healthy Control

Hyperthyroid

vs.

Unpaired t-test:

P>0.05

Unpaired t-test: Euthyroid

vs.

Healthy Control

Authors: Anne Lene Dalkjaer Riis, Jens Otto Lunde Jorgensen, Niels Moller, Jorgen Weeke,and Torben Clausen

Methods

9 Hyperthyroid Patients were tested before and after medical treatment with methimazole

  • 3 Month Grace Period Between Testing

8 Healthy subjects were tested just once

Measurements

Ca2+-ATPase Concentration was determined by Enzyme Activity after purification

Energy Expenditure calculated by indirect calorimetry

Authors Hypothesis Confirmed

  • Ca2+-ATPase Concentration was greater in patients with hyperthyroidism than in healthy patients

  • Ca2+-ATPase levels become normalized after treatment with methimazole

  • That there exists a correlation between Ca2+-ATPase concentration and Energy Expenditure

Presentation by: Christina Clermont and Nik Mohan

Examine Relationships Between

  • Thyroid Function and Cation-ATPase Activity (Concentration)

Hypothesis: In hyperthyroid subjects the contents of Ca(2+)-ATPase in skeletal muscle are increased, and restored toward normal level following standard treatment of hyperthyroidism

  • Cation-ATPase Activity (Concentration) and Energy Expenditure

Hypothesis: The concentration of Ca2+-ATPase in skeletal muscle are correlated to Resting Energy Expenditure

Importance

Purpose

Discussion

Background

Shows that Thyroid function is related to the concentration of CA2+-ATPase in Human Skeletal Muscle

Skeletal Muscle Tested: Vastus Lateralis Muscle

  • The concentration of Ca2+-ATPase in patients with hyperthyroidism is signifcantly greater than in healty patients (p=0.0005)

Thyroid hormone increases the mRNA expression and protein synthesis of Ca2+-ATPase

Tested For: Concentration of Ca2+-ATPase,

and Energy Expenditure

Study the affect that Thyroid Function has on Cation Pumps located in Human Skeletal Muscle

Review: Thyroid Function

9 Patients with Hyperthyroidism

Groups Tested:

Increase in Ca2+-ATPase = Faster Ca2+ Cycling in muscles

Review:Cation Pumps in

Human Skeletal Muscle

- Studied Once Before and Once After Treatment with Methimazole

(Euthyroid)

  • The concentration of Ca2+-ATPase in patients with hyperthyroidism before treatment is significantly greater than after treatment with methimazole (p=0.04)

Part of Endocrine System

- Produce, Store, and Release Hormones

Compared Against

Calcium (Ca2+) pump

Ca2+ transport in skeletal muscle may be a regulatory target for energy turnover and weight control

Reason:

To see if a relationship exists between Thyroid Hormones, Energy Expenditure, and Amount of (Ca2+)-ATPase in Skeletal Muscle

8 Healthy Subjects

- Enzyme: Ca2+-ATPase

- Located on Sarcoplasmic Reticulum (SR)

Thyroid Hormones:

- Triiodothyronine (T3)

- Thyroxine (T4)

- More Enzyme = Greater Turnover Rate

  • There exist a signifcant positive correlation between Ca2+-ATPase concentration and Energy Expenditure (p=0.03)

(SERCA)

Sarco(endo)plasmic Reticulum Ca2+ -ATPase

Controls Metabolism!

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