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Hyperthyroidism and Cation Pumps in Human Skeletal Muscle
Results
Hyperthyroid: 6,555 +/- 604 pmol/g
Euthyroid: 5,212 +/- 1,580 pmol/g
Results
Healthy Control:
4,523 +/-1,311 pmol/g
vs.
Euthyroid
Hyperthyroid
Paired t-test:
Healthy Control
Hyperthyroid
vs.
Unpaired t-test:
Unpaired t-test: Euthyroid
vs.
Healthy Control
Authors: Anne Lene Dalkjaer Riis, Jens Otto Lunde Jorgensen, Niels Moller, Jorgen Weeke,and Torben Clausen
Methods
9 Hyperthyroid Patients were tested before and after medical treatment with methimazole
- 3 Month Grace Period Between Testing
8 Healthy subjects were tested just once
Measurements
Ca2+-ATPase Concentration was determined by Enzyme Activity after purification
Energy Expenditure calculated by indirect calorimetry
Authors Hypothesis Confirmed
- Ca2+-ATPase Concentration was greater in patients with hyperthyroidism than in healthy patients
- Ca2+-ATPase levels become normalized after treatment with methimazole
- That there exists a correlation between Ca2+-ATPase concentration and Energy Expenditure
Presentation by: Christina Clermont and Nik Mohan
Examine Relationships Between
- Thyroid Function and Cation-ATPase Activity (Concentration)
Hypothesis: In hyperthyroid subjects the contents of Ca(2+)-ATPase in skeletal muscle are increased, and restored toward normal level following standard treatment of hyperthyroidism
- Cation-ATPase Activity (Concentration) and Energy Expenditure
Hypothesis: The concentration of Ca2+-ATPase in skeletal muscle are correlated to Resting Energy Expenditure
Importance
Purpose
Discussion
Background
Shows that Thyroid function is related to the concentration of CA2+-ATPase in Human Skeletal Muscle
Skeletal Muscle Tested: Vastus Lateralis Muscle
- The concentration of Ca2+-ATPase in patients with hyperthyroidism is signifcantly greater than in healty patients (p=0.0005)
Thyroid hormone increases the mRNA expression and protein synthesis of Ca2+-ATPase
Tested For: Concentration of Ca2+-ATPase,
and Energy Expenditure
Study the affect that Thyroid Function has on Cation Pumps located in Human Skeletal Muscle
Review: Thyroid Function
9 Patients with Hyperthyroidism
Groups Tested:
Increase in Ca2+-ATPase = Faster Ca2+ Cycling in muscles
Review:Cation Pumps in
Human Skeletal Muscle
- Studied Once Before and Once After Treatment with Methimazole
(Euthyroid)
- The concentration of Ca2+-ATPase in patients with hyperthyroidism before treatment is significantly greater than after treatment with methimazole (p=0.04)
Part of Endocrine System
- Produce, Store, and Release Hormones
Compared Against
Ca2+ transport in skeletal muscle may be a regulatory target for energy turnover and weight control
Reason:
To see if a relationship exists between Thyroid Hormones, Energy Expenditure, and Amount of (Ca2+)-ATPase in Skeletal Muscle
8 Healthy Subjects
- Located on Sarcoplasmic Reticulum (SR)
Thyroid Hormones:
- Triiodothyronine (T3)
- Thyroxine (T4)
- More Enzyme = Greater Turnover Rate
- There exist a signifcant positive correlation between Ca2+-ATPase concentration and Energy Expenditure (p=0.03)
(SERCA)
Sarco(endo)plasmic Reticulum Ca2+ -ATPase
Controls Metabolism!