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Photosynthesis Rate in Green Plant vs. Colored Light

Hypothesis

If leaf disks are exposed to different colored lights, then the rate of photosynthesis will increase or decrease depending upon the colored light.

Null Hypothesis

If leaf disks are exposed to different colored lights, then the rate of photosynthesis will not change.

Dependent and Independent

The dependent variable in the lab was the rate of photosynthesis. The independent variable was the colored light shining on the leaves.

Objective

Does the color of light have an effect on the rate at which 50% of the leaf disks will rise? Also, will the green light cause any of the leaf disks to rise and if so does this mean that some of the green light is being used during photosynthesis?

Experimental Design

Drop the leaf disks that have had all gas removed into the large cups filled with the solutions. Begin timing and record each time a disk rises to the top of the solution up until the 5th disk has risen. This will provide the data needed to determine an ET 50.

Experimental Design

It is important to make sure that you keep the leaf disks that have had the gas removed stay in the dark up until you are ready to start the trial, or else they will start photosynthesising from the light of the room. Place 2 transparent plastic sheets of each color; red, green and blue over 6 of the cups. Leave the remaining two cups as the control and don't place any sheets over those cups. In order to assure that the each trial will receive the same amount of light, have the lamp's hover 7 in. over the surface of the desk. Assign one lamp to each cup.

Experimental Design

Begin setting up the apparatus by filling 8 cups with 200 mL of water. Fill each cup with .4g of baking soda and 1 drop of soap from a pipet. Cut out 10 leaf disks per cup of water. Insert each set of leaf disks into a syringe. Use the syringe to suck up 10 mL of the water solution in the cup. Use the syringe to remove all the gas from the air spaces within the disks. This will be accomplished when the disks float to the bottom of the syringe.

The rate of the disks rising vs. the colore of light

Not all of the date that was obtained will be used in graphing the ET 50. The data that was chosen is

Green: 4:20

Red: 4:14

Blue: 3:39

Clear: 3:18

Data Summery and Explanation

Color of Light Key:

V= Violet

I=Indigo

B=Blue

G=Green

Y=Yellow

O=Orange

R=Red

Patter Explanation

The data that was created from the lab preformed was a very close to what was expected based on how ROYGBIV light effects the rate of photosynthesis in green plants. The only results that came as a surprise what the data that the green light was providing. Green light should have produced an even slower rate of photosynthesis than what was produced in the experiment. The blue light had the highest rate of photosynthesis, which matches what should happen, and red light had a slower rate than blue and a faster rate than green which is also right where the rate should be.

Color of light

Within the graph which is provided on two slides previous, there are generalized data points that were not tested but have a line through them. This is referring to any data points going through V,I,Y, and O. A line is able to be assigned to a general point within these areas because there is definitive data saying what rate photosynthesis should be occurring for these colors. If Violate, Indigo, Yellow and Orange were to be tested within this lab, their date should fall within the generalized points which are expressed in the graph.

The rate of the disks rising vs. the color of light

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