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Transcript
Setting up
the Lab
VAT130 - Laboratory Procedures
Rachel Kinser RVT, VTS(ECC)
Temperature
Location
Designing
the
Laboratory
Storage
Lighting
Design
Tabletops
Internet
Safety
Concerns
Safety
OSHA
OSHA
- United States Department of Labor
- Your right as an employee
Equipment
Safety Equipment
- Eye wash stations
- Spill clean up kits
- Hazardous waste disposal
- PPE
- Gloves
- Goggles
- Face shields
SOPs
Standard Operating Procedures
- Laboratory procedures manual
- Clear instructions
Obtaining the
Sample
Sample
Collection
- Sample type?
- Sample size?
- Materials needed?
- Sampling Handling?
Materials
Materials Needed
- Syringe size
- 22 g or larger needle
- Blood tube
- Vacutainer
What tube for what test??
Red Top Tube
Additive:
- NONE!
Sample Type:
- Serum
Test:
- Chemistry
Blue Top Tube
Additive:
- Sodium Citrate
Sample Type:
- Whole blood
Test:
- Coagulation Panel
Green Top Tube
Additive:
- Lithium Heparin
Sample Type:
- Whole blood or plasma
Test:
- Chemistry
- Blood gas
- PCV/TS
Purple Top Tube
Additive:
- EDTA
Sample Type:
- Whole blood or plasma
Test:
- CBC
- PCV/TS
- Blood smear
Collection
Location
Collection Location
- Feline
- Jugular vein – large sample
- Medial saphenous vein – small sample
- Canine
- Jugular vein – large sample
- Cephalic vein or lateral saphenous – small sample
- Large Animals – jugular Vein
- Birds – wing vein
- Rabbits – ear vein
- Laboratory animals – tail vein, infraorbital sinus
Labeling
Labeling Test Tube
Date & Time
Owner's Name
Patient's Name
Patient's Clinic ID #
Handling
Errors
Handling Errors
- Hemolysis
- Hypoglycemia
- Hyperglycemia/Hyperlactatemia
- Changes to cell morphology
- Prolonged coagulation times
Laboratory Math
- Reagent Solutions
- Prepared
- Diluted
- Samples
- Measured
- Diluted
- Corrected Results
- Correct dilution results
Laboratory
Math
Ratios
Dilutions
Dilution Ratio Equations
(Sample amount * Dilution) - sample amount = Diluent amount
Result * Dilution factor = Accurate Result
Sample Amount : Total Volume
Ratio = Fraction = Decimal
- 1:2 = 1/2 = 0.5
1: 2 = 1 Parts to 2 Parts
1:4 dilution of 0.5 ml urine
= (0.5 * 4) - 0.5
= 2 - 0.5
= 1.5
0.5 ml Urine into 1.5 ml NaCl
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