Bovine lymph nodes

7- Bovine granulomatous lymphadenitis

6- Mucormycosis in a calf

Histopathology

History

Histopathology:

Gross pathology:

The presence of fungal hyphae.

A: Characteristic granulomatous lesions in the mediastinal lymphnode.

B: Culture medium with growth of colonies of Mycobacterium bovis.

C: Granulomatous lesions characteristic of tuberculosis.

D: PCR positive results to M. tuberculosis complex.

Tracheobronchial granulomatous lymphadenitis in a 12 months old cattle.

Two nodular lesions were observed, with a granulomatous appearance in the lung and caudal mediastinal lymph node( lung).

confirmed that it was granulomatous lesions but showed the presence of non septate, irregular fungal hyphae within the lesions, some of them inside multinucleated giant cells. Groccot staining confirmed this finding.

A Mucormycosis (formerly known as zygomicosis) is a rare disease, it is not a contagious one,

mucorales are found in the environment and can enter through respiratory airways (also digestive or through wounds in the skin).

A: Tracheobronchial lymphnode with miliary granulomatous lesion, compatible with tuberculosis. In the microbiological culture Nocardia spp. was isolated

B:  Pyogranulomatous lesion characteristic of a bacterial infection

5- Nontuberculous tuberculous lesions in a calf

Diagnosis:

Gross pathology:

Differential diagnosis:

Histopathology:

PCR for the M.tuberculosis complex gave a negative result,

The origin of this mycobacterium could be poultry and the location of the lesions is indicative of a digestive entry. 

M.avium is considered a non-tuberculous mycobacterium because it does not cause tuberculosis in humans

Multiple granulomatous lesions in the mesenteric lymph nodes.

Suspected diagnosis of bovine tuberculosis

lesions of granulomatous lymphadenitis

compatible with bovine tuberculosis, were observed, and Ziehl Neelsen's staining.

Outline

Granulomatous lesions compatible with tuberculosis in the mesenteric lymph nodes of a calf.

Granulomatous inflammatory infiltrate with structures compatible with fungal hyphae.

Microphotograph of Mycobacterium tuberculosis

Pathological Findings in Bovine Lymph node

1- Pyogranulomatous lymphadenitis due to Actinobacillus lingneieresii

2- Granulomatous-looking lesion in a Calf – Nocardia spp

3- Nodular pyogranulomatous lesion on the face of a bovine

4- Sporadic bovine lymphoma in calves

5- Nontuberculous tuberculous lesions in a calf

6- Mucormycosis in a calf

7- Bovine granulomatous lymphadenitis

4- Sporadic bovine lymphoma in calves

Gross pathology:

Adult cattle (mostly around 3 years old)

Presented by: ALRaiyan Al Mukhaini and Nardin Al Kindi

Presented to Dr. Haitham Ali

Characterized by enlarged lymph node.

Usually affects the liver and spleen

(hepatomegaly and splenomegaly).

An enteric form described (usually involving the abomasal mucosa).

TYPES OF

LYMPHOMA

Histopathology:

Causative agent:

Neoplastic cells infiltrate the nerve sheath and enter the epidural fat, compressing the spinal cord.

(neoplastic lymphoid proliferation)

lymphoma may be caused by the enzootic bovine leukosis virus.

Diagnosis:

Sporadic bovine lymphoma.

1- The calf sporadic bovine lymphoma: can appear at birth or around 6 months of age. Generalized lymphadenopathy and leukaemia.

The kidneys are often affected, and the liver, spleen and thymus may be involved or not.

1- Pyogranulomatous lymphadenitis due to Actinobacillus lignieresii in a cow

Histopathology

Gross pathology:

2- Juvenile form: mainly affects the thymus.

3- Cutaneous form: more common in animals that are two years old.

15-year-old cross-bred cow

2- Granulomatous-looking lesion in a Calf

3- Nodular pyogranulomatous lesion on the face of a bovine

1- bacterial pyogranulomatous lymphadenitis (intralesional bacteria)

2- Splendore Hoeppli

Hence: bovine tuberculosis ruled out

Unilateral increase in the size of the retropharyngeal and prescapular lymph nodes.

When sectioned yellowish and slightly mineralized lesions generated

Suspected for bovine tuberculosi

Gross pathology:

Histopthology

Gross pathology

Splendore Hoeppli (Truyers et al. 2014)

A granulomatous-looking lesion in the caudal mediastinal lymph node of a calf.

chronic piogranulomatous stomatitis (inflammation of the mouth), highly compatible with a case of wooden tongue, an infection by A. lignieresii.

Diagnosis

Microbiology and Gene sequencing

Histopathology:

A 3-year-old female, friesian breed cow

A nodular lesion in the left cheek ulcered the skin and had a cauliflower aspect (10x10 cm in diameter).

From the oral cavity, a soft tissue proliferation above the left upper gum.

Submandibular and parotid lymph nodes were enlarged

Culture:

Presence of neutrophilic polymorphonuclear leukocytes and the visualization of filamentous structures within the cytoplasm of giant cells was indicative of a bacterial etiology. 

The presence of giant cells: indicates tuberculosis but they do not usually appear in such big amounts.

Ziehl Neelsen's staining gave a negative result.

Culture: Abundant colonies of gram-positive bacteria compatible with Nocardia spp. a cause of environmentally derived mastitis in cattle and small ruminants.

Culture: very abundant growth of a bacteria phenotipically compatible with  Pasteurella pneumotropica.

To confirm:

Gene encoding 16S ribosomal RNA was sequenced: Actinobacillus lignieresii.

A. lignieresii:

causative agent of wood tongue.

some cases of lymphadenitis in bovine, characteristically, is associated with the histological phenomenon of Splendore hoeppli observed in this case.

Abundant growth of Trueperella pyogenes. A bacterium usually associated with the presence of abscesses.

Differential diagnosis:

Abundant multinucleated giant cells could be observed

Abundant neutrophilic polymorphonuclear leukocytes.

Pyogranulomatous lymphadenitis with areas of necrosis.

neoplastic lesions such as a papilloma or squamous cells carcinoma