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Metabolic engineering of P.Pastoris for myo-inositol production by dynamic regulation of central metabolism

Ângela Faria, Cristina Sousa, Maria Cardoso, Vânia Dias

UC: Bioquímica II, 2022/2023

Objetivos

Objetivos

  • Aumentar a produção de inositol produzido por P. Pastoris a partir da regulação da distribuição de fluxo de carbono

  • Estudar a influência das condições de hipoxia na produção de inositol

Métodos

1- Seleção das estirpes e crescimento em meio de cultura

2- Clonagem de DNA (GS115)

3-

4-

5-

Resultados

1- Metabolic engineering of the native inositol biosynthesis

pathway and transport system in P. pastoris

2- Inositol production by slowing down the carbon fux

of glycolytic pathway

3- Enhancement of inositol production by introduction of

heterologous ScIPS and EcIMP

4- Further improvement of inositol production by dynamic

regulation of glycolysis and pentose phosphate pathways

5- High ‑cell‑ density fermentation with strain JQ08

1

Metabolic engineering of the native inositol biosynthesis

pathway and transport system in P. pastoris

Substituição do promotor do gene IPS por pGAP

Estirpe JQ01

Estirpe JQ02

Fermentação:

  • produção de etanol
  • sem produção de inositol

Remoção dos transportadores PpITR1 e PpITR2

2

Inositol production by slowing down the carbon fux

of glycolytic pathway

Click to edit text

3

Enhancement of inositol production by introduction of

heterologous ScIPS and EcIMP

Click to edit text

4

Further improvement of inositol production by dynamic

regulation of glycolysis and pentose phosphate pathways

Click to edit text

5

High ‑cell‑ density fermentation with strain JQ08

Click to edit text

Topic 4

Discussão

Subtitle 1

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