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1- Seleção das estirpes e crescimento em meio de cultura
2- Clonagem de DNA (GS115)
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1- Metabolic engineering of the native inositol biosynthesis
pathway and transport system in P. pastoris
2- Inositol production by slowing down the carbon fux
of glycolytic pathway
3- Enhancement of inositol production by introduction of
heterologous ScIPS and EcIMP
4- Further improvement of inositol production by dynamic
regulation of glycolysis and pentose phosphate pathways
5- High ‑cell‑ density fermentation with strain JQ08
Substituição do promotor do gene IPS por pGAP
Estirpe JQ01
Estirpe JQ02
Fermentação:
Remoção dos transportadores PpITR1 e PpITR2
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