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Cell Respiration Lab- Gabby P, Gabby V, & Chloe De Palo
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Cell Respiration Lab
Gabby Puchall, Gabby Viggiano, & Chloe De Palo
By Gabby P, Gabby V, and Chloe
Analysis
Questions
Analysis
Pre-lab
Why is it necessary to correct the readings of the respirometers containing seeds with the readings taken from respirometers containing only glass beads? Your answer should refer to the concepts derived from the general gas law: PV = nRT
Why is it necessary to correct the readings of the respirometers containing seeds with the readings tak...
The glass beads will not respirate, making them the constant for the experiment so the data can be referred back to them.
The glass beads will not respirate, making th...
What happens to the volume of the gas being measured (O2 consumption or CO2 production)
when the temperature or pressure changes during the experiment?
What happens to the volume of the gas being measured (...
During O2 consumption if temperature increases during the experiment, and the O2 would exapand. Along with that an increase in pressure would result in a change of volume.
During O2 consumption if temperature increas...
If pressure and temperature remain constant, will the volume of gas in the respirometers
increase or decrease? Please explain.
If pressure and temperature remain constant, wi...
If the pressure and temperature remain constant the volume of the gas in the respirometers decrease. This is because due to the consumption of oxygen the pressure decreases inside the respirometers, and this is what causes water to move into the pipette.
If the pressure and temperature remain consta...
Imagine that you are given 25 germinating pea seeds that have been placed in boiling water
for five minutes. You place these seeds in a respirometer and collect data. Predict the rate of
oxygen consumption (i.e., cellular respiration) for these seeds and explain your reasons.
Imagine that you are given 25 germinating pea seeds th...
The rate of oxygen consumption would decrease, for even though at hotter temperatures there is an increase in consumption, there is a point where it gets too hot and results in the denaturization of the enzymes that aid cellular respiration.
The rate of oxygen consumption wou...
Imagine that you are asked to measure the rate of respiration for a 25g reptile and a 25g
mammal at 10°C. Predict how the results would compare, and justify your prediction.
Imagine that you are asked to measure the rate ...
The mammal would have a faster rate of respiration at 10*C. This is due to the fact that mammals are warm blooded and require internal body temperature to stay the same (which is warmer than 10*C) vs a reptile, which is cold blooded, and do not need to keep a stable internal temperature.
The mammal would have a faster rate of respiration ...
Imagine that you are asked to repeat the repti...
Imagine that you are asked to repeat the reptile/mammal comparison of oxygen consumption,
but at a temperature of 22°C. Predict how these results would differ from the measurements
made at 10°C, and explain your prediction in terms of the metabolism of the animals.
In comparison to 10*C, at 22*C the reptile would consume a lot more energy than it did in the lower temperature. This is due to the fact that eating increases kinetic energy in cells by speeding up the molecules involved in chemical reactions which means cellular respiration would happen faster in the reptile and that leads to the consumption of more oxygen.
In comparison to 10*C, at 22*C the reptile would consu...
What difficulties would there be if you used a living gre...
What difficulties would there be if you used a living green plant in this investigation instead
of germinating seeds?
A living green plant would carry out the process of photsynthesis, producing oxygen, making the consumption of oxygen off when the measurment was taken from the pipette.
A living green plant would carry out the process ...
Bolded Questions
What specific things could you measure?
What specific things could you measure?
In this lab the consumption of oxygen will be measured, a...
In this lab the consumption of oxygen will be measured, and the second lab we measured the output of carbon dioxide.
Which of these might be easier or harder to measure?
Which of these might be easier or harder to measure?
The consumption of oxygen is easy to measu...
The consumption of oxygen is easy to measure because KOH can be used to make sure no carbon dioxide is released messing up the data.
What factors can affect the rate of respiration?
What factors can affect the rate of respiration?
1. Temperature
2. Pressure
(as mentioned in the pre-lab questions)
1. Temperature
2. Pressure
(as mentioned in the pre-l...
Analysis Questions
What evidence did you obtain to suggest the peas are performing cellular respiration? Explain.
What evidence did you obtain to suggest the peas a...
The graph below shows evidence that the germinati...
The graph below shows evidence that the germinating peas are perfroming cellular respiration, for the line increases meaning it has a positve slope and that shows the releasing of carbon dioxide which is given off as a waste product during the process of photosynthesis.
What is the effect of germination on the rate of cell respiration in peas? Explain why your data
make biological sense. In other words, why would germination have an effect on the rate at
which these organisms are performing cellular respiration?
What is the effect of germination on the rate of ce...
It is necessary for germinating seeds to undergo cellular respiration in order to retain the energy they need for growth and development. Unlike full grown pea plants, the seeds do not yet have the necessary photosynthetic abilities needed to produce their own energy sources.
It is necessary for germinating seeds to under...
Relative to the peas germinating at ro...
Relative to the peas germinating at room temperature, what would you expect to happen to the
rate of CO2 production if you had placed 25 germinating peas on ice for 10 minutes before
collecting your data? Explain.
The rate of carbon dioxide production would not be ...
The rate of carbon dioxide production would not be as fast, for coming from ice before the experiment is conducted means the peas would not be producing as much energy since they are cold (dormant) leading to less production of carbon dioxide.
data
O2 Concentration Over Time
O2 Concentration Over Time
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Rate of Oxygen Consumption per Minute
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Rate of Co2 Production by 25 non-germinating Peas (ppm/sec)
Rate of Co2 Production by 25 non-germinating Peas (ppm/sec)
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Rate of CO2 Production by 25 Germinating Peas (ppm/sec)
Rate of CO2 Production by 25 Germinating Peas (ppm/sec)
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Designed experiment
Designed experiment
Question being Investigated
Does the temperature of germinating seeds affect the rate of cellular respiration?
Plant cells are required to respire in order to produce energy to maintain order, grow, and reproduce. Cellular respiration changes chemical energy into usable energy for the cells. Changes in the environment can affect the rate of respiration of the plant cells, including a change in temperature. At certain temperatures enzymes and proteins within a cell can denature, leading to no cellular respiration, while other temperatures offer an environment where the enzymes and proteins can fucntion at their maximum efficiency. Each type of seed has its own optimal temperature, In order to test this problem three beakers cotaining germinating seeds will be placed in different temperatures and the rate of repiration will be measured using a conductivity probe. The rates of respitation at a cold tempature, a room temperature, and a warm termperature will provide the necessary data on how temperature affects the cellular respiration. The control of the experiment will be the germinating seeds at room temperature.
Hypothesis
If tmperature affects the rate of respiration of cells, then if germinating seeds are placed in a hotter environment than optimal, then the rate of respiration will decrease and have eventually no repiration, and when placed in a colder environment, then the rate of respiration will be low but will still occur.
When cells are in a temperature higher than the optimal temperature for respiration, the proteins and enzymes within the cell may begin to denature, damaging them, decaresing the rate of repiration since it can no longer respirate. When in a colder environment, the molecules will move slower, so the rate of respiration will be slow but still occur.
Variables
Variables
The independent varibables are the different temperatures the germinating seeds are placed in.
The dependent variable is the rate of repiration of the germinating seeds in each environment.
The control is the rate of respiration of the gerninating seeds at room temperature and the amount of seeds used in each experiment.
The temperatures will be controlled using a thermometer and the amount of germinating seeds will be counted before each experiment.
Materials
- Computer
- LabQuest2
- Vernier C02 Gas Sensor
- 3 Erlenmeyer Flasks
- germinating/ growing peas
- 50 mL graduated cylinder
- paper towels
- thermometer
- ice
- bucket
- timer
- heat lamp
Procedure
1. Count out 20 germinating seeds and place them on a paper towel. (Use different geminating peas for each trial).
2. Pour 20 mL into the 50 mL gracdulated cylinder
3. Add the seeds into the cylinder and record the volume by taking the final volume in the cylinder and subtract 20 mL.
4. Place the germinating seeds on a paper towel and then place the seeds into an Erlenmeyer flask.
5. Turn on LabQuest. Under sensors, select "Sensor setup." Next to CH 1, change "no sensor" to "CO2 gas" and then select "CO2 gas old". Click "ok" until there is a screen with red data readouy box. Select the icon that looks like a graph
6. Put the Vernier CO2 gas sensor into the opening of the flask, making sure no air can escape.
7. Measure the CO2 production by clicking the green "play" button. The data will be collected for 10 minutes.
8. Determine the temperature of the environment the seeds will be placed in using the thermometer.
9. Leave the flask in the room temperature (23≥≥≤°C) room and start the 1o minute timer.
10. Determine the rate of respiration by selecting the linear portion of the graph by placing the stylus on the right most data and dragging it left. Black brackets should frame the section selected.
11. Select "Analyze--> curve fit --> CO2 --> Choose fit = linear
12. Record the slope of the line as the rate of respiration.
13. Remove the gas sensor and gently remove the germinating peas.
14. Pour the ice into a bucket and use the thermometer to check the temperature which should be aroud 0°C.
15. Repeat steps 1-8, and instead place the flask into the bucket of ice for 10 minutes and record the data using steps 10-13.
16. Turn on the heating lamp and let it heat to 38°C.
17. Repeat steps 1-8 again, and place the flask under the heat lamp for 10 minutes and record the data using steps 10-13.
16. Compare the rates of respiratio in the higher and lower temperatures to that of the room temperature rate.