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To investigate the effect of Taxol on fungal tip growth in Neurospora Crassa.
What is Taxol?
Taxol is a chemotherapy drug.
microtubules are essential for rapid hyphal growth
Hypothesis (Ha): Taxol has an effect on hyphal tip growth
Null Hypothesis (Ho): Taxol has no effect on hyphal tip growth
1) Set up microscope for Kohler illumination at 40x magnification
2) Obtain the innoculated Neurospora Crassa agar slides
3) Separate 4 slides into two groups; control and experimental.
Experimental: 50uL growth medium with Taxol
Control: 50 uL growth medium
Both were incubated for 25 miniutes.
4) Place experimental slides under microscope. Use Phase-Contrast (Ph2) and align one end of the ocular scale with a hyphal tip and the other away from the tip.
5) Record the growth of the tip every 2 minutes, over a 16 -minute interval.
3 fungal tips were observed in each group.
Figure 1. Average distance travelled by hyphal tip of N. crassa exposed to 50 microlitres of growth medium over a period of 16 minutes. Data shows a linear increase in movement of hyphal tips in control samples with a rate of 0.99 micrometres/minute.
Figure 2. Average distance travelled by hyphal tip of N. crassa exposed to 50 microlitres of taxol over a period of 16 minutes. Data shows a linear increase in hyphal tip growth with a rate of 0.62 micrometres/minute.
The two-tailed P value equals 0.4102.
By conventional criteria, this difference is considered to be not statistically significant because it is greater than 0.05.
How fast do the tips grow?
Control: approximately 0.99 micrometres/minute
Experimental : approximately 0.62 micrometres/minute.
Does each hypha grow at a steady speed or does it vary over time?
Not exactly, but growth increments were fairly similar every 2 minutes over a 16 minute interval.
Do all hyphae grow at the same rate?
No, some hyphae grow faster than others. See experimental data above.
It was predicted that Taxol has an effect on hyphal tip growth in Neurospora Crassa.
Therefore Taxol does have an effect on hyphal tip growth. Suggests that microtubules play a role in hyphal tip growth.