Antimicrobial potential of native vegetal species of Brazilian semi-arid region

National Policy of Medicinal Plants and Phytotherapics (PNPMF, 2006)

National Program of Medicinal Plants and Phytotherapics (PNPMF, 2009)

National List of Medicinal Plants for SUS (RENISUS, 2009): 71 species indicated for use in basic health care

Semi-arid biome (caatinga)

Governmental initiatives - principles

A xeric shrub-dominated biome in Brazil’s northeast (~735,000 Km²);

Low altitudes, high temperatures, low humidity, annual rainfall (250-500 mm) concentrated in 2-3 months;

A peculiar kind of the vegetation adapted to the prevailing local ecological conditions;

Supports a hight, yet poorly studied, diversity of plant resources.

Expansion of therapeutic options in SUS (Public Health System of Brazil);

Sustainable use of the Brazilian biodiversity;

Appreciation and preservation of traditional and folk knowledge.

Brazil

Introduction

Antimicrobial potential of native vegetal species of Brazilian semi-arid region

Biological and ethnomedicinal importance: >55.000 vegetal species and many are used in folk medicine.

Kátia Mayumi Takarabe Caffaro

M.Sc. Student

Graduate Program in Nursing

Federal University of Alagoas State / Brazil

Endemic species of Caatinga

University/Laboratory location

Sampling location

The seasons of caatinga

Alagoas State

Evaluate the antibacterial activity of two endemic vegetal species - Bauhinia cheilantha (Bongard) Steudel and Lippia gracilis Schauer - from semi-arid brazilian region used as antiinfective agents in a folk medicine.

Objetive

Winter

Brazil

LpTF: Research Laboratory of Wound Treatment

Search of species with antimicrobial activities for the treatment of wounds.

In vitro assays → in vivo assays

Other in vitro assays: interdisciplinarity

10 species sampled based on an ethnopharmacological study that listed 187 vegetal species

Popular use as antiinfective agents

Dry season (lasts 7 months or more)

L. gracilis extracts and S. aureus

Etanolic extracts (10%)

Diffusion method in solid media

Confection of wells with 7 mm diametres;

Bacterial suspensions (106 CFU/mL) in Mueller Hinton Agar;

50 µL of plant extract /well, in a concentration of 10%;

Negative controls: DMSO and Tween 80 (10%);

Positive controls: Ceftriaxone (30 µg) and/or Ciprofloxacin (5 µg);

Incubation at 36°C, 24h.

In vitro experimental study

Diffusion method in solid media

ATCC strains used: Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae

Triplicates

Methodology

Results and discussion

C-

C+

5

6

Etanol 96%

(5): etanolic extract of aerial parts of L. gracilis

(6): etanolic extract of stem of L. gracilis

(C -): Tween 80

(C +): standard antibiotic

Tween 80 or DMSO (10%)

in

Saline solution

Measurement of inhibition using a caliper rule

B. cheilantha extracts and S. aureus

Lippia gracilis

Bauhinia cheilantha

2

3

Other bioassays

Evaluate the antifungal activity

Minimum Inhibitory Concentration - MIC

Free radical scavenging capacity

Cytotoxicity by cell viability

Chemical characterization of the components responsible for the biological activity and toxicological analysis

The results indicate the presence of compounds with antimicrobial activity in both species.

Other tests are still needed to support its use in folk medicine.

Conclusions

5

4

C-

6

(2): etanolic extract of leaves of B. cheilantha

(3): etanolic extract of stem bark of B. cheilantha

(4): etanolic extract of stem of B. cheilantha

(C -): DMSO

Muito obrigada!

Thank you!

Muchas gracias!

Delmiro Golveia city

Caffaro, KMT

Universidade Federal de Alagoas / UFAL

Laboratório de Pesquisa em Tratamento de Feridas / LpTF

mayumikatia@gmail.com

Maceió city