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Mode of Antimicrobial Action of Clove Essential Oil Against the Foodborne Pathogen Campylobacter jejuni

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Two-dimensional gel electrophoresis (2D SDS-PAGE)

Clove EO induced changes in proteome

FIG. 3. Differences in the protein expression patterns of the control (A.) and clove EO treated (B.) C. jejuni 11168 were compared by 2D PAGE analysis. Numbers represent differently expressed proteins.

Clove EO induced changes in proteome

FIG. 2. Protein chip-based electropherograms of clove EO-treated (dashed line) and untreated (continuous line) NCTC 11168 strain.

Step 2

Minimal inhibitory concentration and minimal bactericidal concentration of clove EO against C. jejuni

Effect of clove EO on the motility of C. jejuni

Table 1. Aligned migration times, molecular weights, and areas belonging to the dominant peaks presented in Figure 2.

FIG. 1. Effect of clove EO in 1: 3 000 dilution on the proliferation of C. jejuni strain NCTC 11168.

Judit Klára Kovács

Effect of clove EO on C. jejuni morphology

Department of Medical Microbiology and Immunology, University of Pécs, Hungary

FIG. 4. Scanning electron micrographs of non-treated (A, B) and clove EO treated (1: 3 000) (C, D) C. jejuni cells. Untreated C. jejuni showed the typical slightly curved spiral morphology, while clove EO treated cells became shortened and less intensely curved.

III. Interdisciplinary Doctoral Conference 2014

Clove (Syzygium aromaticum)

www.wadsworth.org

www2.vmas.kitasato-u.ac.

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