DNA Structure
Chemical Properties
- Alternating Backbone
- Phosphate and Sugar Group
- 4 Nitrogenous Bases
- thymine-adenine(2 hydrogen bonds)
- guanine-cytosine(3 hydrogen bonds)
- Bonds
- Phosphodiester, hydrogen, and glycosyl Bonds
- Anti-Parallel
- 5' and 3' ends
DNA Replication
Initiation
- Semi-conservation
- Parent and Daughter Strand
- Origins of Replication
- Replication Fork and Bubble
- Enzymes
- DNA Helicase
- DNA Gyrase
- Single Stranded Binding Proteins(SSBs)
- Anneal and Re-anneal
Lagging Strand
Leading Stand
- Continuously synthsized
- One RNA Primer
- Towards Replication Fork in the 5'to 3 prime direction
- Discontinuously Synthesized
- Many RNA primers
- Away from Replication Fork in the 3'to 5'direction
- DNA Polymerase 3
- DNA Polymerase 1
- DNA Ligase
- Okazaki Fragments
Physical Properties
The diameter of DNA is 2nm
DNA Structure and Replication
- The helix makes one complete turn every 10 nucleotides.
- The distance between nucleotides is 0.34nm
- One complete turn accounts for 3.4nm
DNA Structure
DNA stands for DeoxyriboNucleic Acid
and its in the shape of a double helix
Emma Ratia
Termination
- DNA polymerase 1 and 3
- Proofreading
- Mistakes
- Exonuclease
March 11th 2015