The Effect of Caffeine in Mice

only a sample to show that

weight and locomotion increases while memory retention decreases in the effect of Caffeine

CH4 |Data and Results

Mice in Cage A had a significant weight gain, took longer time to complete the maze test and had larger frequency of wheel usage. Mice in Cage C had similar results with Cage B for the first 3 weeks, with Cage A for the remaining weeks.

Cage A (Caffeine)

Cage B (Water)

Cage C (Water + Caffeine)

CH3 | Methodology

This research required 9 Albino mice, 3 transparent cages with a running wheel, liquid dispenser and food containers, a weighing scale and a maze.

Observing Locomotive

Observing Weight

Analysis

Observed during a specific time every day through a thorough observation (by each assigned researcher) of the frequency of the running wheel usage

The researchers compared the results of the tests during a specific time of the day, A with B, then A and B with C.

The weight of each mouse was measured every week, during the first day of the observation week.

Monitoring

Observing Memory

Usage of the maze - first trial had the goal of finding a spot where food was placed, second trial - the mouse was placed on a different starting point. This was done every week as well.

The mice were marked each by A1, A2, A3, B1, B2, B3, C1, C2, C3 D1, D2 and D3. A for Cage A, B for Cage B, C for Cage C. They were fed at 9:10, 12:00 and 4:10 – 5:00. The mice were tested after a three-week continuous routine.

SotP | SotS | SaL

Intro | Background of the Study

Statement of the Problem |

This research intends to test if the specific tea type to be tested enhances locomotion, weight gain, and memory retention.

SaL

Why white mice?

: anatomy and physiological composition is similar to a human’s

: maintainable and managable

: conducted SY 2013-2014

: determine effects of black tea on 3 factors on mice

: does not deal with plants

SotS

: Black

: 42 mg of caffeine per 8 fl. Oz cup

White Mice + Tea

Factors to be observed

The researchers will be able to conclude the negative and positive effects that the chosen type of tea can cause.

Benefactors: caffeine consumers

Locomotion

(dizziness or loss of focus

+ hyperactivity)

Weight

(pre and post experimentation)

Memory retention

(after x days of tea consumption)

CH3 | Research Design

Parallel group design | the mice were divided by A and B where A were the ones who received tea for liquid consuming while B were the ones who received water

The Statistical Instrument

ANOVA which stands for analysis of variance.

The researchers checked whether all the means are equal by examining two estimates of the variance.

CH5 | Conclusion and Recommendation

CH2 | RRL Local

According to Malaya.com, it is advisable to limit consumption of caffeine to more than 500 to 600 milligram s a day.

Filipinobook.com

Conclusion

Recommendation

Caffeine equivalent to 5 cups of coffee per day reduced the build-up of destructive plaques in the brain of mice.

Mice were more likely to develop disorders that often lead to type 2 diabetes.

Talakayanatkalusugan.com

Caffeinated coffee to mice that were engineered to develop Alzheimer’s disease either had the disease progression slow, or the disease never developed at all.

There were significant differences in the results per cage. The caffeine indeed induced weight gain, less effective memory and higher levels of locomotion compared to water. It was as they predicted in their hypotheses.

To all, especially to those fond of sodas, frappes and other forms of caffeine because of the high consumption of various age groups of caffeinated drinks.

They also suggest that a study be made using humans as test subjects to confirm the findings from this study.

Relevance

CH2 | RRL Int'l

: Allison Aubrey (2010), a daily caffeine habit may help protect against Alzheimer's disease

This study expressed results that may help modify the choices consumers make for healthier living and lifestyle.

LiveStrong.com, taking up too much caffeine, can constrict the blood vessels and increase the symptoms of vertigo, the state of dizziness and motion sickness.

Susan Perry (2009), caffeine has its health risks, linked to an increased risk of high blood pressure and, for pregnant women, miscarriages

University of Western Australia also found out that too much caffeine leads to abnormal retention of fat cells, leading to weight gain.

Ch2 | RRL

Conceptual Framework

Research Hypotheses

Definition of Terms

The researchers predicted that weight will increase, memory retention will decrease, and locomotion levels will increase.

: Caffeine – stimulant drug found in tea, coffee, etc; its effects are insomnia, wakefulness and increased heart rate

: Locomotion – act of self-propulsion to be able to survive

: Hyperactivity – physical state where a person is abnormally active

Intro | Background of the Study

Caffeine

PROs

About Caffeine

CONs

: caffeinism

: adverse health effects if consumed excessively

: improves mental attentiveness

: mood-lifting effects

: medical purposes

: naturally-occurring

chemical

: coffee, chocolates,

soda, tea

The Effect of Caffeine in Mice

Group 8 IV-1

Based on Jim Harvey's speech structures