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Transcript

The Effects of Temperature on Coacervate Formation

Results Analysis

The test tube left unaltered saw a very small change in its number of coacervates, as expected. Also as expected, the test tube placed into the hot water bath saw a significant increase in the number of coacervates. However, the test tube placed in the ice bath, contrary to expectations, also saw a significant increase in the number of coacervates present.

Conclusion

Materials

This means that my hypothesis is not, in fact, supported by my data. It would seem that both heat and cold, as opposed to just heat, are conditions that are favorable in terms of coacervate formation. However, this lab experiment was not all encompassing, and only had one trial, so it would be interesting to see what the results would have been had I been able to spend more time on this experiment.

Procedures Cont.

Test Tubes-3

Thermometer-1

Test Tube Caps-3

Microscope Slide-1

Graduated Cylinder-1

Ice Bath-1

Hot Water Bath-1

Cover Slips-6

Pipette-1

Gelatin Solution

Gum Arabic Solution

Then, I placed test tube two in the ice bath and test tube three in the hot water bath. After five minutes passed, I checked the temperature and the number of coacervates again in each tube. Test tube one, which was merely left in the room, had, for some reason, fallen to 13 degrees, and contained 21 coacervates. Test tube two, at 7 degrees C, contained 57 coacervates. Test tube three, at 29 degrees C, contained 100 coacervates.

Question and Hypothesis

Question: What effect does temperature have on the formation of coacervates?

Hypothesis: If the temperature of the solution is increased, then the number of coacervates will increase.

Procedures

Each of my three test tubes had gum arabic solution and gelatin solution placed in them. I immediately measured the temperature in each of them, and then counted the number of coacervates at that temperature. Test tube one, at 24 degrees C, contained 18 coacervates. Test tube two, at 26 degrees C, contained 22 coacervates. Test tube three, at 23 degrees C, contained 14 coacervates.

Variables

Independent: Temperature of the water.

Dependent: Number of coacervates.

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