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ISOLATION OF THEOBROMINE FROM COCOA

Caguimbal, Yvonne R. & Sablaon, Vienna Louise U.

Outline

Results and Discussion

- Background and Objectives

- Methodology

- Results and Discussion

- Conclusion

- References

Background / Introduction

Figure 2.1 Murexide Test

Figure 2.2 Dragendorff'sTest

SAMPLE 1 - InJoy Cocoa

Mass of sample : 10g

Mass of isolate : 0.05 g

Percent yield : 0.5%

SAMPLE 2 - Riccoa

Mass of sample : 10g

Mass of isolate : 0.11 g

Percent yield : 1.1%

Murexide Test

Sample 1- Positive; appearance of purple color

Sample 2- Positive; appearance of purple color

Figure 1.1 Theobromine structure

- Theobromine was discovered by a Russian chemist Alexander Vosresenskey.

- Formerly called as xantheose is an alkaloid belonging to methylxanthines.

- The structure of theobromine is similar to the structure of caffeine

Dragendorff's Test

Sample 1- Positive; appearance of orange pink precipitate

Sample 2- Positive; appearance of orange pink precipitate

Figure 1.2 Caffeine Structure

COCOA

• top source of antioxidants

• it contains Magnesium and Iron

• In terms of medical applications, it can heal wound quickly, it helps to improve cardiovascular health,

• high blood pressure, cholesterol, obesity and constipation.

28 grams of cocoa contains 60 mg theobromine while the same amount of dark chocolate contains 200 mg.

Objectives

Results and Discussion

1. To isolate theobromine from two

different brands of cocoa

2. To qualitatively analyze the compound

through analytical techniques

Standard Curve

Concentration Absorbance

200 0.054

300 0.133

400 0.157

500 0.24

650 0.395

700 0.45

Methodology

LOD Theoretical

(3 x std.dev)/slope = (3 x 0.15)/(7.82x10^-4)

= 575

LOD Theoretical > LOD experimental

T- TEST

(357.065-200)/(46/(√6))

= 8.36

8.36>6.869

tcalc>ttab

ANOVA

P-value

0.5154>0.265

Pcalc>Ptab

F-TEST

5.9874<19.3

Fcalc <Ftab

Conclusion

Reference

• Phytochemicals. (n.d.). Retrieved February 7, 2017,

from http://www.phytochemicals.info/phytochemicals/theobromine.php

• Przybyl, A. K., & Kurekk, J. (n.d.). Laboratory of Organic Chemistry (Natural and Pharmaceutical) (J. Milecki, Ed.).

• Health Benefits of Cocoa. (n.d.). Retrieved March 7, 2017, from https://www.organicfacts.net/health-benefits/other/health-benefits-of-cocoa.html

• Applied Science BTEC Nationals/Chemical Laboratory Techniques/Caffeine Extract. (n.d.). Retrieved from https://en.wikibooks.org/wiki/Applied_Science_BTEC_Nationals/Chemical_Laboratory_Techniques/Caffeine_Extract

-Isolation through reflux

-Final product was white precipitate

-Percent yield: Injoy Cocoa (0.5%) Riccoa ( 1.1%)

-Chemical test: Positive in Dragendorff's test and Murexide test

-T-test: standard is comparable to the sample

-LOD: method was considered to be accepted

Possible source of error:

-Refluxing Time

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