Yeast Sphere Catalase Lab
Erin Oliver, Allison James, Andie Stokes
Results
Our Data Chart
(Sorry it's a bit messy but hey that's science for you)
How we measured the temperature of the hydrogen peroxide solution
Introduction
- Question our experiment answers: Does the increase of temperature of the hydrogen peroxide solution affect the rate of reaction in the yeast spheres?
- Hypothesis: If the temperature of the solution is increased, then the rate of reaction increases.
Discussion
Abstract
- The results that we acquired were much compared to the online activity that we did. When we slowly increase the temperature the reactions did happen faster until we got to 61 degrees where there was no reaction at all
- we received the result that we because when you heat up a solution the particles or enzymes are moving fast and create products at a quick rate until its too fast where the enzymes just stop making products
- Our hypothesis: If the temperature increases, then the rate of reaction increases.
- Our hypothesis i proven to be correct until it got up to 61 degrees where the reactions just plain stopped
- the next studies that we would be conducting is mesuring the reaction rate when the temperature gets colder
Methodology
Does the increase in temperature of solution increase or decrease the rate of reaction in the yeast spheres?
- Materials: Yeast spheres, beakers, hot plate, thermometer, graduated cylinder, water, hydrogen peroxide, CACL2, yeast, sodium alginate
In our experiment, we tested how the rate of reaction of the yeast spheres would react in a warmer solution of hydrogen peroxide. First, we measured the temperature of the solution at room temperature, which was 21 degrees Celsius. Then, we heated up the solutions 10 degrees higher at a time. To calculate the best results, we did 3 trials for each temperature. We tested 5 different temperatures; 31 degrees, 41 degrees, 51 degrees, 61 degrees, and 71 degrees.
Our results showed that the rate of reaction increased up until the 51 degree solution. But, once we tested the 61 degree solution the rate of reaction slowed down tremendously. While the other trials took 4-13 seconds for the yeast spheres to float to the top, the 61 degree trial took 3 minutes. Once we tested the 71 degree solution, the reaction never took place, which made the rate of reaction 0.
- Procedure: Make the yeast spheres, heat water of yeast spheres and H2O2 to the temp wanted, stick one of the yeast spheres into the warmed H2O2, watch when the reaction starts, count the seconds it takes for the yeast sphere to reach the top for each trial and temperature.
- Any statistical tests performed to indicate the reliability of our results: Doing the experiment with 3 trials to test it, yeast sphere lab