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The Golden Age of Microbiology (1800s-1900s)

1. Does Microbial Life Spontaneously Generate

Living things arose from three processes

Experiment 1 for Spontaneous Generation

1. Asexual Reproduction

2. Sexual Reproduction

3. Nonliving matter proposed spontaneous generation

Redi Experiment (meat and maggots)

4. Living things can arise from nonliving matter (abiogenesis)

Decaying meat kept isolated from flies, maggots never developed

meat exposed to flies was infested

Doubted Aristotle

Experiment 2 of Spontaneous Generation

Needham Experiment

Large animals not spontaneous but microbes are

Boiled Gravy and plant materials then sealed vials , vials became cloudy

Explanation- life forced caused heated matter to come to life

Experiment 3 of Spontaneous Generate

Spallanzani Experiment

Concluded on Needham finding

Needham failed to heat vials enough to kill all microbes (so they contaminate)

Spontaneous does not occur,all living things arise from other living things

sealed vials do not allow enough air for organisms to survive

4 Questions of Life

1. Is spontaneous generation of

microbial life possible?

2.Is spontaneous generation of microbial life possible?

3.What causes disease?

4.How can we prevent infection and disease?

Microbial Life Spontaneously Generate Cntd

Experiment 5

Pasteur Experiment

Perfomed same experiment as Virchow but with Swan necked flasks

Flask were upright and no growth happened

Experiment 4

Rudolf Virchow (pathologist)

Introduced Biogenesis- the synthesis of substances by living organisms.

Living cells can only arise from pre existing living cells

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