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The Effect of Temperature on the Osmosis of Water into a Tea Bag

Jenna Cook & Alexandria Brown

Introduction and Hypothesis

Sources Cited

UA Little Rock, Department of Biology, editor. Science of Biology Laboratory Manual. UA Little Rock, 2017.

Conclusion

All tea bags gained a significant amount of weight- an average of 31 grams- between 0 and 10 minutes. The tea bag in Beaker 3 gained a constant weight of about 1 gram per minute between 10 and 30 minutes. The weight of all tea bags reached their peak weight at 30 minutes, and dropped an average of about 3 grams between 30 minutes and 40 minutes. An error could have occurred with Beaker 2 in the Hot Water Bath. The temperature was warming up, and did not reach 47 degrees Celsius until towards the end of the experiment, so the temperature of Beaker 2 fluctuated considerably throughout the first 20-30 minutes of the experiment. The hypothesis did not hold up. Even though this could have been due to the severe fluctuation of temperature in Beaker 2, it cannot be confirmed. Beaker 3, the coldest beaker, had the most constant, and fastest rate of osmosis.

The effect that temperature has on the osmosis of water into a tea bag was measured for the "Design Your Own Experiment" project. The hypothesis was that the beaker with the highest temperature of water- Beaker 1 at 47 degrees Celsius- would have the fastest rate of osmosis, and the beaker with the lowest temperature of water- Beaker 3 at 20 degrees Celsius- would have the slowest rate of osmosis. This is because molecules tend to move faster at higher temperatures.

Background

According to the Science of Biology Laboratory Manual, osmosis is the movement of water across a cell membrane from areas of low solute concentration (hypotonic) to areas of high solute concentration (hypertonic) (Biology Dept. of UA Little Rock, 2017).

Procedure

First, measure the weight of each tea bag before starting the experiment. Next, put 500 mL of water in each of the 3 beakers, labeling them 1,2, or 3. Leave Beaker 1 at room temperature with one tea bag. Put Beaker 2 in the 47 degree Celsius hot water bath with one tea bag. Put Beaker 3 in the refrigerator with one tea bag, and record the temperature. Then measure the weight of the tea bags every 10 minutes by placing them each on a weigh boat, then onto the scale. Replace the tea bags as quickly as possible. Be sure to measure the weigh boat prior to the beginning of the experiment and subtract its weight from the total to get the weight of the tea bag, or zero the scale with the weigh boat.

Materials

1. 4 Beakers of 1,000 mL volume

2. 1 Hot Water Bath at 47 degrees Celsius

3. 1 Refrigerator

4. 3 Tea Bags weighing 8 grams each

5. Water

6. 2 Glass Thermometers

7. Digital Scale

8. 3 Weigh Boats

Data Table

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