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Pyruvate and Succinate in Cellular Respiration

What is cellular respiration?

Cellular respiration is a series of metabolic processes that provide energy for organisms via the conversion of biochemical energy obtained through consumption of organic material into ATP that can be used by the cell.

Cellular Respiration is aerobic, meaning that it requires oxygen to occur.

The Citric Acid cycle occurs in the mitochondrial matrix.

What is pyruvate?

Fun Facts

As a supplement it is used for treating obesity, high cholesterol, cataracts, and cancer.

Role in Respiration

-End product of glycolysis

-Converted to Acetyl CoA

-Can be broken down into lactate(animals) or ethanol(plants) in anaerobic environment.

Fun Facts:

As a supplement it is used for various purposes ranging from the treatment of menopause symptoms, skin care, arthritis, and joint pain!

What does it do?

-Succinate is involved in the 6th step of the citric acid cycle

-The enzyme succinate dehydrogenase catalyzes the removal of two hydrogen from succinate

-FAD is reduced to FADH2 as it takes the hydrogens from succinate

What is succinate?

Research Question

How does succinate and pyruvate differ in their impact on cellular respiration?

Hypotheses

Prediction

If pyruvate is involved in the first step of the citric acid cycle, then increased levels of pyruvate will show higher levels of cellular respiration than increased levels of succinate

Pyruvate and succinate are both involved in the citric acid cycle, but considering pyruvate is one of the initial reactants within the cycle, it follows that the presence of pyruvate will reveal a higher level of impact on cellular respiration than succinate.

Results

Procedure

Conclusion

Both succinate and pyruvate impact cellular respiration, which was not suprising to see in the results since we knew going into the experiment that both are naturally present in the citric acid cycle. The new variable tested was the pyruvate, which as discussed/shown in the graphs is less effective at increasing cellular respiration than the originally tested succinate. The main question that arose from this experiement is whether or not varying the levels of pyruvate and succinate would provide a different conclusion about which was more succesful at altering the rate of respiration. For instance, if we tested 0.3 mL of each, and then 0.6mL etc it would be interesting to see if there was a change in trends.

Experiment Procedure

Experimental Procedure

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