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by Rustam Bensalem on 5 December 2012

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#### Transcript of Radish Seed Germination Experiment

Germination Experiment Observing the effects of different pH levels Experiment We analyzed the germination of radish
seeds after watering them with solutions of
varying pH levels. For the first sample
we used a milk solution (2% milk and baking soda), which is very basic and had a
pH of 10. For the Second sample we used
lemon juice, which is very acidic with a pH
of 3. We then conducted a control
experiment, using bottled water, with a
neutral pH of 7. We predicted that both the
basic and acidic samples will
inhibit some form of germination,
with the more acidic sample to
outlast the basic sample.
We also predicted the control to have
the most biomass, followed by the
acidic sample, then the basic, after
the 5 day period. In addition to this we
believe that each solutions will maintain
the same pH during this 5 day span. Hypothesis Materials 3 Petri Dishes
Paper towels (cut to size)
40 mL water
40 mL 2% milk
40 mL pure lemon juice Rustam Bensalem, Andrew Snigur, Mihail Mihaylov
Austa Somivichian-Clausen Methods Line each petri dish with two
paper towels creating a bilayer.
This retains liquid, and speeds
up the germination process by
keeping the shell of the seed
soft. Step 1... Step 2... Step 3... Saturate each dish as follows:
First dish with 20mL of milk solution. (pH= 10)
Second dish with 20mL of lemon juice. (pH= 3)
Third dish with 20mL of bottled water. (pH=7) Step 4... Appropriate ten seeds into each sample petri dish. Situate the dishes in a well lit area and observe for 5 days. Ensure that each solution maintains the same pH. On the second day, add the remaining 20mL of each solution to their respective petri dishes. Day 1 Day 5 Control Milk solution (ph 9) Lemon Juice
(pH 3) Day 3 Control Control Milk solution (ph 9) Milk solution (ph 9) Lemon Juice
(pH 3) Lemon Juice
(pH 3) DATA Initial Biomass Final Biomass Change Acidic
Sample Control
Sample Basic
Sample Number of Seeds Germinated # of seeds pH levels: 5 day span # of seeds Results H O W E V E R ... Possible Errors The data shows that our hypothesis was accurate in some ways and inaccurate in others. The milk and baking soda solution actually decreased in pH levels, and the coinciding sample showed no sign of germination We were correct, however, in predicting that the acidic sample would show some germination. Although it was shortlived the seed did respond to this solution Lack of sunlight
UV light as opposed to sunlight.

The fat and oils in the milk blocked poors in the seeds coating, stopping germination.

Bacterial colonies that formed as a result of the milk.
Bacteria would ultimately deprive the seed of any nutrient or minerals it could derive from milk.

Too much/little solution fed to plants

Temperature
Too cold Upon measuring the overall biomass in each sample, apart from the control, the acidic sample was the only to increase reflecting that som germination did infact occur. Conditions Seeds can only germinate and sustain growth
with a solution that has a pH between 5 and 8. Radish seeds typically germinate in 3/4 days Require temperatures between roughly 60-80
degress fahrenheit.
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