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How does pH affect enzyme function?

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by Lorina Harvey on 18 October 2013

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Transcript of How does pH affect enzyme function?

How does pH affect enzyme function?
Results
The results show that the yeast exposed to a neutral solution released more CO2 than the yeast exposed to the acidic and alkali solution.
What are enzymes?
Method
Purpose
The purpose of this experiment is to investigate the affect that pH has on enzyme function.

What was observed?
The experiment showed that the enzymes in neutral conditions had more enzyme activity than the other two conditions.

It also showed that the enzymes in the alkaline conditions had very low activity.
What can be concluded?
It can be concluded that the neutral clearly displayed a better level of activity than the enzymes in the lower and higher pH conditions. Therefore, the hypothesis can be accepted.

How was this done?
Water displacement was measured to determine the amount of enzyme activity that occurred.
Enzymes are substances that are produced by living organisms. They act as catalysts and help to speed up reactions.
Effects of pH
Enzymes are affected by changes in pH. The most favorable pH value - the point where the enzyme is most active - is known as the optimum pH. This is graphically illustrated below:
Hypothesis
Enzymes in the neutral conditions will have a higher level of activity.
Just a quick word before the Method begins...
This investigation was carried out to test the effect(s) of varying an abiotic factor, p.H, on enzyme function in yeast. Three different p.H’s were tested, including an acidic solution, a neutral solution, and an alkaline solution. Hydrogen peroxide was the substrate which the enzymes aided in breaking down, and the level of water displaced/ oxygen collected was used to determine how active the enzymes were.
Alkalinity
The third condition tested was alkaline, with the specific p.H being arbitrary. Again, a water bath was set up with an inverted beaker connected to a length of tubing via a bung. This was connected to the test tube in which was placed 5ml of hydrogen peroxide, 0.6 grams of baker’s yeast, and 5ml of sodium hydroxide. A timer was started when the mixture began reacting. The reaction was stopped by disconnecting the tubing at the two minute mark, and then the water displacement level was measured in the beaker.
Acidity
The first condition tested was acidic, with the specific p.H being arbitrary. A water bath was set up with an inverted beaker connected to a length of tubing via a bung. This was connected to the test tube in which was placed 5ml of hydrogen peroxide, 0.6 grams of baker’s yeast, and 5ml of hydrochloric acid. A timer was started when the mixture began reacting. The reaction was stopped by disconnecting the tubing at the two minute mark, and then the water displacement level was measured in the beaker.
Neutrality
A neutral solution was the second type of condition tested. The same water bath was set up with an inverted beaker connected to a length of tubing via a bung. This was connected to the test tube in which was placed 5ml of hydrogen peroxide, 0.6 grams of baker’s yeast, and 5ml of distilled water. A timer was started when the mixture began reacting. The reaction was stopped by disconnecting the tubing at the two minute mark, and then the water displacement level was measured in the beaker.
http://www.worthington-biochem.com/introbiochem/effectsph.html
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