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Light vs. Electron Microscope

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by Ruth Ati on 13 September 2012

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Transcript of Light vs. Electron Microscope

Light Microscope Pros of Light Microscope Feature/Benefits contd. Features/Benefits Purpose of the Light Microscope Cons of the Light Microscope Limited Magnification. Can only magnify up to 1,000 times. No light microscope can magnify more than 2,000 times.

Limited depth of field. Light microscopes provide only 2-dimensional images which causes an unclear image of the object/organism being studied. Has more than one lens which makes it easier to see details of an object/organism.

The high intensity fluorescence light from the light microscope helps magnify images for better clarity of the object/organism being viewed. Ocular lens- magnifies objects ten times the normal size.

Objective lens- the three to four lenses attached to the nose piece that changes the magnification.

Light source- the light bulb under the stage that directs light from the rotating disk under the stage to the viewers eyes. Adjustment Knobs: light microscopes usually have two knobs that adjust the height of the stage and the focus of the object/organism. Also known as the the fine adjustment knob and the coarse adjustment knob. The light microscope is used to detect small objects using visible light and lenses.

Can be used to magnetize living or dead samples up to 1000 times the original size. Bibliography Perkins, Bryan. (April 16, 2012). Features of a Compound Light Microscope. In Ehow Tech. Retrieved September 11, 2012, from http://www.ehow.com/list_6009143_features-compound-light-microscope.html. Kennedy, David. (April 16, 2012). The Advantages of a Light Microscope. In Ehow Tech. Retrieved September 11, 2012, from http://www.ehow.com/about_5070922_advantages-light-microscope.html.

Banas, Timothy. (n.d.). The Disadvantages of Using Light Microscopes. In Ehow . Retrieved September 11, 2012, from http://www.ehow.com/list_6195820_disadvantages-using-light-microscopes.html. Kenney, Caitlin. (n.d.). What is a light microscope?. In wiseGeek. Retrieved September 11, 2012, from http://www.wisegeek.com/what-is-a-light-microscope.htm. Electron Microscope The purpose of an electron microscope is to be able to study structure and cells in greater detail than than with a light microscope or the naked eye. Pros of Electron Microscope. Electron microscopes have much greater resolving power than light microscopes and can obtain much higher magnifications. Some electron microscopes can magnify specimens up to 2 million times, while the best light microscopes are limited to magnifications of 2000 times
Electron microscopes provide us with the greatest magnification (over 100,000x) and resolution.
Depending on the microscope, it is possible to view the three dimensional external shape of an object. Features & Benefits Cons of Electron microscope. Electron microscopes are extremely expensive.
space requirements are high. they need a whole room.
They are difficult to use.
they do not possess color.
•The energy of the electron beam is very high. The sample is therefore exposed to high radiation, and therefore not able to live. Has a higher magnification or resolving power than a normal light microscope.
Electron microscopes therefore allow for the visualization of structures that would normally be not visible by optical (light) microscopy Bibliography Electron Microscopes vs. Optical (Light) microscopes. (n.d.). MicrobeHunter.com - Microscopy Magazine and Blog. Retrieved September 12, 2012, from http://www.microbehunter.com/2009/01/22/electron-microscopes-vs-optical-light-microscopes/



Electron microscope. (n.d.). Science Daily: News & Articles in Science, Health, Environment & Technology. Retrieved September 12, 2012, from http://www.sciencedaily.com/articles/e/electron_microscope.htm Similarities between Light and Electron Microscopes They both enable us to see small objects that cannot be seen through to the naked eye.
They both have objective lens which is used in magnifying and enlarging a image
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