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Copy of Practical Parasitology 2

Intestinal and Urogenital Protozoa
by

Eslam Medhat

on 20 April 2013

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Transcript of Copy of Practical Parasitology 2

Practical Parasitology Lab 2 Classification of Human Parasites Protozoology Study of protozoa (primitive animals) Helminthology Study of helminths (worms) Entomology Study of arthropods Blood and tissue Intestinal tract (a) Amoeba: Entamoeba histolytica
(b) Intestinal flagellates: Giardia lamblia
(c) Intestinal ciliate: Balantidium coli
(d) Intestinal sporozoa: Cryptosporidium parvum Site of infection Species Urogenital tract Hemoflagellates: Trypanosoma sp, Leishmania sp.
Blood Sporozoans: Plasmodium sp.
Tissue Sporozoans: Toxoplasma gondii Urogenital flagellate: Trichomonas vaginalis I. Classification of pathogenic protozoa according to the site of infection II. Classification of pathogenic protozoa according to mode of locomotion & reproduction Rhizopoda
(Amoebae)
- Pseudopodia
- Asexual reproduction by binary fission Ciliata
(ciliates)
- Cilia
- Asexual reproduction by transverse binary fission Flagellata
(flagellates)
- Flagella
- Asexual by longitudinal binary fission Sporozoasida
(Sporozoa)
- Non-motile.
- Asexual and sexual reproduction Multiply encyst mature cyst pass in feces ingestion
(food, water...) In small intestine (Giardia, Cryptosporidium)
In large intestine (Ent., B. coli) Trophozoite Excystation A. Intestinal protozoa (General life cycle) Type of protozoan (amoeba, ciliate, flagellate: intestinal or blood & tissue, sporozoan).
Disease caused by protozoan.
Infective stage.
Lab diagnosis (diagnostic stage).
Form of protozoan under the microscope.
Vector for the protozoan.
Location (site of infection) of the protozoan in the body. Questions form Entamoeba histolytica Amoebic dysentry or amoebiasis E. histolytica trophozoite minuta form. 10-20 µm in diameter. Trichrome stained film.
(Diagnostic stage) E. histolytica trophozoite: in wet mount preparation
(diagnostic stage) E. histolytica trophozoite: iodine stained.
(diagnostic stage) Trophozoite:
Active, feeding and motile form with amoeboid outline, one nucleus: with central large black chromatin granule called Karyosome, and nuclear membrane is lined with very small chromatin granules. E. histolytica cyst: iodine stained.
(Infective and diagnostic stage) E. histolytica trophozoite magna form (dysenteric). 12-25 µm in diameter, with ingested erythrocytes in the endoplasm. Trichrome stained film.
(Diagnostic stage) Cyst: resistant , infective form, round or oval, contains from 1-4 nuclei. Usually 4 in mature cyst and black chromatoid bars. Type of protozoan: amoeba
Disease caused by protozoan: amoebic dysentery
Infective stage: cyst
Diagnostic stage: cyst or trophozoite in stool*.
Forms of the protozoan: trophozoite or cyst
Location in the body: large intestine (intestinal) and in liver, lung or brain (extraintestinal amoebiasis). 1- Entamoeba histolytica Balantidium coli Balantidial dysentry or balantidiasis * videos B. coli trophozoite wet mount preparation showing cilia
(Diagnostic stage) B. coli trophozoite wet mount preparation showing cilia
(Diagnostic stage) B. coli cyst: showing cilia and arrow directed to the cytostome. Trichrome stain
(Infective and diagnostic stage) Largest protozoan parasite in human.
Trophozoite: oval, covered with cilia, kidney shaped nucleus. B. coli trophozoite wet mount preparation showing cilia
(Diagnostic stage) B. coli cyst (infective and diagnostic stage) B. coli trophozoite showing the large kidney shaped nucleus
(Diagnostic stage) B. coli cyst (infective and diagnostic stage) Type of protozoan: ciliate
Disease caused by the protozoan: Balantidial dysentery
Infective stage: cyst
Lab diagnosis (diagnostic stage): trophozoite or cyst in stool*
Forms of the protozoan: trophozoite or cyst
Location in the body: large intestine 2- Balantidium coli Giardia lamblia Giardiasis Giardia lamblia trophozoite in stool (Diagnostic stage) Giardia lamblia trophozoite in stool (Diagnostic stage) Giardia lamblia trophozoite in stool (Diagnostic stage) Giardia lamblia trophozoite in stool (Diagnostic stage) Giardia lamblia cyst (infective and diagnostic stage) Giardia lamblia cyst (infective and diagnostic stage) Giardia lamblia cyst (infective and diagnostic stage) Giardia lamblia cyst (infective and diagnostic stage) Type of protozoan: flagellate
Disease caused by the protozoan: Giardiasis
Infective stage: cyst
Lab diagnosis (diagnostic stage): trophozoite or cyst in stool
Forms of the protozoan: trophozoite or cyst
Location in the body: small intestine Giardia lamblia Trichomonas vaginalis B. Urogenital Protozoa Trichomoniasis -Pear shaped, 1 nucleus, 5 flagella: 4 free, 5th attached by an undulating membrane, axostyle is present and has cytostome (mouth). Trichomonas vaginalis trophozoite: with four anterior flagella and a fifth flagellum on the margin of the short undulating membrane. Trichomonas vaginalis trophozoite: undulating membrane and axostyle are apparent. (diagnostic and infective) Trichomonas vaginalis trophozoite (diagnostic and infective) Trichomonas vaginalis trophozoite (diagnostic and infective) Type of protozoan: urogenital flagellate
Disease caused by the protozoan: trichomoniasis
Infective stage: trophozoite.
Lab diagnosis (diagnostic stage): trophozoite in vaginal or urethral discharge in females and in anterior urethral or prostatic secretion in males.
Forms of the protozoan: trophozoite only.
Location in the body: urogenital tract of male (urethra and prostate) and female (vagina). Trichomonas vaginalis * video
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