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TESTING THE EFFECTIVENESS OF HOUSEHOLD DISINFECTANTS

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by

Elly Kackerman

on 2 December 2013

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Transcript of TESTING THE EFFECTIVENESS OF HOUSEHOLD DISINFECTANTS

TESTING THE EFFECTIVENESS OF HOUSEHOLD DISINFECTANTS ON COMMON BACTERIA
THE OBJECTIVE:
-To analyze how commercial cleansing wipes inhibit bacterial growth
THE BASICS
Bacterial Candidates
Escherichia coli
Bacillus megaterium
Staphylococcus simulans
Procedure using sterile techniques:
Cultured the bacteria at 37 degrees Celsius in liquid agar
Dipped swab into bacteria and then rubbed on a 6 inch by 6 inch sterile cutting board surface
Wiped the infected surface with a selected disinfectant wipes
Swabbed surface and vortexed into 5 mL of buffered peptone water
Transferred 1 mL of bacterial remnants onto new TSA plate and spread
Cultured the bacteria at 37 degrees Celsius for approximately 24 hours and analyzed growth via colony counting
Repeated 5 trials for each type of wipe with each strain of bacteria
Disinfectant Candidates
Lysol Disinfectant Wipes
Pure Power Green Disinfectant Wipes
Clorox Disinfectant Wipes
WHAT WE USED
126 swabs
63 sterile test tubes
63 petri dishes (TSA)
Sterile buffered peptone water
37 degree incubator
Micropipettors
Ethanol
Cultures ofthe 3 Bacteria
The three types of disinfectant wipes
DESIGN
In order to complete the goal we would have to :
- Have a control to prove bacteria viability
- Have a reproducible method to see how effective each wipe was at killing microorganisms.
-Limit variables in tests to types of bacteria and the type of wipe
-Minimize contamination by using sterile supplies throughout experiment
MARC
ADELEN
SARAH
PATRICK
37 C
12/2/13
A. onthisproject
Introduction
Purpose : To test the claim that by using disinfectant wipes a surface becomes sterile because of claims that they can kill 99.9% of germs. We believe Clorox wipes will be the best.


The experiment is significant because it puts the claims of the companies to the test.
RESULTS
Discussion
The results show that the green disinfectant wipe, overall, inhibited the growth of the bacteria more than Lysol and Clorox, but they were all effective at killing most of the bacteria.
The Lysol wipes however were very effective at killing S. simulans.
These results are unusual because common sense would suggest the typical unrestrained wipes would be effective than the wipes that compete in effectiveness but are restricted to safer chemicals.
OUTSIDE SOURCES
To prove that the bacteria used were viable and would proliferate on their own, a control group was tested by going through the process without wiping the surface at all. The results of these tests were all too numerous to count, but it proved that the bacteria would grow if unaltered.
An experiment completed by Beth M. Layton of Saint Martin's University, involved
Salmonella
bacteria and Clorox, Lysol, and Safeway brand disinfectant wipes.
Her procedure involved measuring zones of inhibition and were with 12 trials each, but her results indicated that Lysol wipes were better than Clorox which was better than Safeway.
The control was with water, which was only slightly less effective than Safeway.
Discussion (Continued)
The original hypothesis that Clorox wipes will be the most effective wipes was not supported by the results of the experiment.
However, the claim that wiping off surfaces with the disinfectant wipes kills almost all of the microorganisms was supported
Another unexpected result was the contamination of one set of trials.
Bibliography
1. Beth M. Layton. 2006. Disinfectants and Salmonella: A Study Showing the Effectiveness of Disinfectants Against the Bacteria Salmonella. Saint Martin’s University Biology Journal of May 2006, Volume 1. 95-103. http://advancedbio-treatment.com/blog/wp-content/uploads/2011/11/Salmonella-Disinfectant-1.pdf
2. Jean Fleming. 2008. What You Really Need to Know About Surface Disinfectant Efficacy Claims. Infection Control Today. http://www.infectioncontroltoday.com/articles/2008/05/what-you-really-need-to-know-about-surface-disinf.aspx
3. University of Alberta Faculty of Medicine and Dentistry. 2011. Comparison of wiping away bacteria with disinfectant wipes or a tissue moistened with saline. Science Daily. http://www.sciencedaily.com/releases/2011/03/110316152937.htm

Possible Explanations
According to one study done by a team of researchers in the Faculty of Medicine & Dentistry at the University of Alberta, bacterial counts drop the more often a plate is swiped regardless of wipe. For instance, swiping 3 times compared to 1 time reduced bacterial load by 88%.
Disinfectant wipes proved to be more effective than a wipe with saline solution with one swipe, but by 3 swipes they had equal effectiveness.
Also, according to another source, in order for a product to be consider a disinfectant, it must be tested and proven to control organisms that pose a threat to human health. Such products are tested specifically for
Salmonella enterica
,
Staphylococcus aureus
,
and
Pseudomonas aeruginosa
.
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