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AP Biology: Leaf Discs Photosynthesis Lab

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Prerana Ramadurgum

on 10 March 2013

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Transcript of AP Biology: Leaf Discs Photosynthesis Lab

Foundation Conclusion Background Graphs Data Analysis Table It was anticipated that the solution with the most carbon dioxide would have the highest rates of photosynthesis. When there is more carbon dioxide in the surrounding environment, more carbon can be fixed and photosynthesis can occur faster. The control with water took the longest time and did not have more than half of its leaf discs rise within 30 minutes. There is not much carbon dioxide in water, therefore photosynthesis occurs very slowly. There was also not much different seen between the .2% and the .4% . Their lines seem to criss-cross on the graph and they almost have the same rate while the .6% has a much higher rate and a more distinct line. This could mean that substrate below .6% was not enough for all the enzymes to have a substrate to react on. In .6%, the activity only seems to pick up. The next step would be to test .8% and onwards until enzymatic reactions seem to go down. The rate of photosynthesis actually seemed to be picking up around the 9th and 10th leaf, this could mean another experiment with more than 10 leaves should follow to see how much higher the rate will increase and when it will finally start declining. Photosynthesis Lab The rate of photosynthesis can be measured in two different ways: the disappearance of substrate or the amount of products produced. This experiment deals with measuring the amount of oxygen accumulated. Leaves usually contain CO2 and O2 and therefore they float on water. However, if the carbon dioxide and oxygen were removed the leaves would sink. Then, as photosynthesis and respiration occur you can find the net rate of photosynthesis occurring in a leaf over some time. However, once these leaves are put in a vacuum and all the oxygen and CO2 is gone, they still need CO2 to perform photosynthesis so they are put in a solution of bicarbonate so they can get CO2 from the environment. The results seem to follow what was thought to have happened. The more carbon dioxide a plant has, the faster the rate of photosynthesis. This is shown when comparing the three different solutions, .2% having the least carbon dioxide and .6% having the most. The .4% and the .2% don't seem to be as set apart as the .6% Experiment In this experiment, disks are punched into spinach leaves and are put in solutions with either water, .2% bicarbonate, .4% bicarbonate, or .6% bicarbonate. Before being submerged in the solution, the leaves are put in a vacuum made with a syringe.The time it takes for the leaves to rise to the surface of their solution will indicate the net rate of photosynthesis. Prerana Ramadurgum
Irvinn Rincon
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